caa a22 4500 463218078 CHVBK 20180405153203.0 cr unu---uuuuu 170326e20070701xx s 000 0 eng 10.1007/s11056-007-9039-6 doi (NATIONALLICENCE)springer-10.1007/s11056-007-9039-6 Role of polyamines during in vitro rhizogenesis of Nothofagus nervosa using successive culture media [Elektronische Daten] [G. Martínez Pastur, M. Arena, M. Benavides, E. Eliasco, N. Curvetto] An in vitro rooting protocol for producing Nothofagus nervosa microplants using successive steps during tissue culture is reported. Qualitative and quantitative changes in putrescine, spermidine, and spermine tissue contents, during the rooting process and their correlation with other biochemical markers and morphological changes, provided the rationale for their use during the study of a successive in vitro culture protocol for N. nervosa, as well as the use of their inhibitors. The polyamine tissue content was maximum before root emergence (spermine raised to 100nmol/g FW to later decreased to ca. 50nmol/g FW in the following days), but it was lower in improved rooting media (197nmol/g FW compared to 264nmol/g FW of the control medium). It was concluded that polyamines qualitatively and quantitatively improve in vitro rhizogenesis, and the best successive culture media included 10μM spermine during the expression phase. Springer Science+Business Media, Inc., 2007 Rooting markers nationallicence Rhizogenesis nationallicence Polyamine inhibitors nationallicence Root system quality nationallicence Marcadores del enraizamiento nationallicence Rizogénesis nationallicence Inhibidores de poliaminas nationallicence Calidad del sistema radical nationallicence Martínez Pastur G. Centro Austral de Investigaciones Científicas (CADIC-CONICET), cc 92, 9410, Ushuaia, Tierra del Fuego, Argentina aut Arena M. Centro Austral de Investigaciones Científicas (CADIC-CONICET), cc 92, 9410, Ushuaia, Tierra del Fuego, Argentina aut Benavides M. Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires (UBA), Junín 956, 1113, Buenos Aires, Argentina aut Eliasco E. Centro Austral de Investigaciones Científicas (CADIC-CONICET), cc 92, 9410, Ushuaia, Tierra del Fuego, Argentina aut Curvetto N. Departamento de Agronomía, Universidad Nacional del Sur (UNSur), San Andrés 800, Altos del Palihue, 8000, Bahía Blanca, Argentina aut New Forests Kluwer Academic Publishers 34/1(2007-07-01), 83-93 0169-4286 34:1<83 2007 34 11056 https://doi.org/10.1007/s11056-007-9039-6 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11056-007-9039-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Martínez Pastur G. Centro Austral de Investigaciones Científicas (CADIC-CONICET), cc 92, 9410, Ushuaia, Tierra del Fuego, Argentina aut NATIONALLICENCE 700 1- Arena M. Centro Austral de Investigaciones Científicas (CADIC-CONICET), cc 92, 9410, Ushuaia, Tierra del Fuego, Argentina aut NATIONALLICENCE 700 1- Benavides M. Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires (UBA), Junín 956, 1113, Buenos Aires, Argentina aut NATIONALLICENCE 700 1- Eliasco E. Centro Austral de Investigaciones Científicas (CADIC-CONICET), cc 92, 9410, Ushuaia, Tierra del Fuego, Argentina aut NATIONALLICENCE 700 1- Curvetto N. Departamento de Agronomía, Universidad Nacional del Sur (UNSur), San Andrés 800, Altos del Palihue, 8000, Bahía Blanca, Argentina aut NATIONALLICENCE 773 0- New Forests Kluwer Academic Publishers 34/1(2007-07-01), 83-93 0169-4286 34:1<83 2007 34 11056 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer