caa a22 4500 463218108 CHVBK 20180405153203.0 cr unu---uuuuu 170326e20070701xx s 000 0 eng 10.1007/s11056-007-9038-7 doi (NATIONALLICENCE)springer-10.1007/s11056-007-9038-7 Regeneration of Melia volkensii Gürke (Meliaceae) through direct somatic embryogenesis [Elektronische Daten] [S. Indieka, D. Odee, G. Muluvi, K. Rao, J. Machuka] Experiments were conducted to study plant regeneration through direct somatic embryogenesis using mature zygotic embryo and cotyledonary explants from seeds of Melia volkensii stored for <3 and >12months. Explants were cultured on Murashige and Skoog (MS) medium supplemented with BAP, NAA and 2,4-D (0.5, 1.0 and 2.0mgl−1) alone, and BAP (0.5, 1.0, 2.0 and 4.0mgl−1) in combination with 2,4-D or NAA (0.2 and 0.5mgl−1). After 4weeks in culture, up to 60% of cotyledonary explants from the seeds stored for <3months produced direct somatic embryos on BAP (0.5-4.0mgl−1) in combination with 2,4-D (0.2mgl−1). The number of somatic embryos ranged from 5 to 14 per explant in BAP (0.5mgl−1) and 2,4-D (0.2mgl−1) combination. Only 20% of cotyledonary explants from seeds stored for >12months produced somatic embryos. Mature zygotic embryos failed to produce any somatic embryos. Subcultures of somatic embryos from cotyledonary explants of seeds stored for <3months formed clusters of shootlets on semi solid MS and 1/2 MS media. After 6weeks of subculture on multiplication MS media augmented with BAP (0.5mgl−1) and IAA (0.2mgl−1), 70% of the shoot tips formed 4-7 shoots per explant. Up to 33% of the multiplied shoots were rooted in MS medium supplemented with 2.0mgl−1 IBA. Plantlets developed normally into seedlings in the greenhouse. Springer Science+Business Media, Inc., 2007 Cotyledon nationallicence Mass-propagation nationallicence Seed storage nationallicence Somatic embryos nationallicence Zygotic embryos nationallicence Indieka S. Biotechnology Laboratory, Kenya Forestry Research Institute, P.O. Box 20412, 00200, Nairobi, Kenya aut Odee D. Biotechnology Laboratory, Kenya Forestry Research Institute, P.O. Box 20412, 00200, Nairobi, Kenya aut Muluvi G. Department of Biochemistry and Biotechnology, Kenyatta University, P.O. Box 43844, 00100, Nairobi, Kenya aut Rao K. International Plant Genetic Resources Institute-Regional Office for Sub Saharan Africa, C/o ICRAF, P.O. Box 30677, 00100, Nairobi, Kenya aut Machuka J. Department of Biochemistry and Biotechnology, Kenyatta University, P.O. Box 43844, 00100, Nairobi, Kenya aut New Forests Kluwer Academic Publishers 34/1(2007-07-01), 73-81 0169-4286 34:1<73 2007 34 11056 https://doi.org/10.1007/s11056-007-9038-7 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11056-007-9038-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Indieka S. Biotechnology Laboratory, Kenya Forestry Research Institute, P.O. Box 20412, 00200, Nairobi, Kenya aut NATIONALLICENCE 700 1- Odee D. Biotechnology Laboratory, Kenya Forestry Research Institute, P.O. Box 20412, 00200, Nairobi, Kenya aut NATIONALLICENCE 700 1- Muluvi G. Department of Biochemistry and Biotechnology, Kenyatta University, P.O. Box 43844, 00100, Nairobi, Kenya aut NATIONALLICENCE 700 1- Rao K. International Plant Genetic Resources Institute-Regional Office for Sub Saharan Africa, C/o ICRAF, P.O. Box 30677, 00100, Nairobi, Kenya aut NATIONALLICENCE 700 1- Machuka J. Department of Biochemistry and Biotechnology, Kenyatta University, P.O. Box 43844, 00100, Nairobi, Kenya aut NATIONALLICENCE 773 0- New Forests Kluwer Academic Publishers 34/1(2007-07-01), 73-81 0169-4286 34:1<73 2007 34 11056 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer