caa a22 4500 463218124 CHVBK 20180405153203.0 cr unu---uuuuu 170326e20071101xx s 000 0 eng 10.1007/s11056-007-9055-6 doi (NATIONALLICENCE)springer-10.1007/s11056-007-9055-6 Petroleum flotation technique upgrades the germinability of Casuarina equisetifolia seed lots [Elektronische Daten] [V. Sivakumar, R. Anandalakshmi, R. Warrier, B. Singh, Mulualem Tigabu, P. Odén] The germination of Casuarina equisetifolia Forst seed lots is low (40-50%) even when freshly collected. This study aimed at upgrading the germination performance by removing non-germinable seeds using petroleum flotation technique. Seeds collected from 19 individual trees in seedling seed orchards in India were assessed using X-ray to determine the proportion of different seed lot fractions. The feasibility of petroleum ether as a separation medium was tested. Morphological characters of floating and sunken seeds were further studied using image analysis technique to examine their effects on the separation efficiency. The X-ray analysis revealed a large quantity of shrivelled, empty and insect-damaged seeds, altogether accounting 50%, which were the causes of low percentage germination of un-graded seed lots (48%). Petroleum flotation resulted in 90% germination in the sunken fraction and 4% in the floating fraction. The separation was distinct for most seed lots, except few seed lots that had relatively low germination in the sunken fractions and more than 10% germination in the floating fractions. As a whole, petroleum flotation appears to be a feasible technique to upgrade the germination of C. equisetifolia seed lots, and its efficacy is influenced by wing surface area, seed density and wing quotient of filled and empty seeds. Springer Science+Business Media B.V., 2007 Empty seeds nationallicence Filled seeds nationallicence Specific density nationallicence Seed lot enhancement nationallicence Sivakumar V. Division of Seed Technology, Institute of Forest Genetics and Tree Breeding, Indian Council of Forestry Research and Education, P. B No. 1061, 641002, Coimbatore, India aut Anandalakshmi R. Division of Seed Technology, Institute of Forest Genetics and Tree Breeding, Indian Council of Forestry Research and Education, P. B No. 1061, 641002, Coimbatore, India aut Warrier R. Division of Seed Technology, Institute of Forest Genetics and Tree Breeding, Indian Council of Forestry Research and Education, P. B No. 1061, 641002, Coimbatore, India aut Singh B. Division of Seed Technology, Institute of Forest Genetics and Tree Breeding, Indian Council of Forestry Research and Education, P. B No. 1061, 641002, Coimbatore, India aut Tigabu Mulualem Department of Forest Genetics and Plant Physiology, Seed Laboratory and Tropical Silviculture Group, Swedish University of Agricultural Sciences, 901 83, Umea, Sweden aut Odén P. Department of Forest Genetics and Plant Physiology, Seed Laboratory and Tropical Silviculture Group, Swedish University of Agricultural Sciences, 901 83, Umea, Sweden aut New Forests Springer Netherlands 34/3(2007-11-01), 281-291 0169-4286 34:3<281 2007 34 11056 https://doi.org/10.1007/s11056-007-9055-6 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11056-007-9055-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Sivakumar V. Division of Seed Technology, Institute of Forest Genetics and Tree Breeding, Indian Council of Forestry Research and Education, P. B No. 1061, 641002, Coimbatore, India aut NATIONALLICENCE 700 1- Anandalakshmi R. Division of Seed Technology, Institute of Forest Genetics and Tree Breeding, Indian Council of Forestry Research and Education, P. B No. 1061, 641002, Coimbatore, India aut NATIONALLICENCE 700 1- Warrier R. Division of Seed Technology, Institute of Forest Genetics and Tree Breeding, Indian Council of Forestry Research and Education, P. B No. 1061, 641002, Coimbatore, India aut NATIONALLICENCE 700 1- Singh B. Division of Seed Technology, Institute of Forest Genetics and Tree Breeding, Indian Council of Forestry Research and Education, P. B No. 1061, 641002, Coimbatore, India aut NATIONALLICENCE 700 1- Tigabu Mulualem Department of Forest Genetics and Plant Physiology, Seed Laboratory and Tropical Silviculture Group, Swedish University of Agricultural Sciences, 901 83, Umea, Sweden aut NATIONALLICENCE 700 1- Odén P. Department of Forest Genetics and Plant Physiology, Seed Laboratory and Tropical Silviculture Group, Swedish University of Agricultural Sciences, 901 83, Umea, Sweden aut NATIONALLICENCE 773 0- New Forests Springer Netherlands 34/3(2007-11-01), 281-291 0169-4286 34:3<281 2007 34 11056 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer