caa a22 4500 463218221 CHVBK 20180405153204.0 cr unu---uuuuu 170326e20070901xx s 000 0 eng 10.1007/s11056-007-9045-8 doi (NATIONALLICENCE)springer-10.1007/s11056-007-9045-8 In vitro flowering of green and albino Dendrocalamus latiflorus [Elektronische Daten] [Choun-Sea Lin, C. Liang, H. Hsaio, M. Lin, W. Chang] To propagate Dendrocalamus latiflorus, we used in vivo inflorescences to produce calli on Murashige and Skoog basal (MS) medium supplemented with 3mg/l 2,4-dichlorophenoxyacetic acid (2,4-D), 2mg/l kinetin, 250mg/l polyvinyl pyrrolidone (PVP), and 1% coconut milk. Multiple shoots were generated on MS medium supplemented with 0.1mg/l thidiazuron (TDZ). The green plantlets were successfully transferred to soil. Multiple albino shoots also regenerated and were able to proliferate on medium containing cytokinins, especially TDZ. Albino multiple shoots rooted in medium containing α-naphthaleneacetic acid (NAA), and callus formation was observed in the presence of 2,4-D and picloram. Green and albino regenerates flowered after 8months of subculture. The flowering ratio increased to 44% after three treatments in medium containing 1mg/l TDZ. Morphological observations revealed that the in vitro green and albino flower organs were normal. However, pollen derived from the in vitro flowers of both the green and albino plants were sterile. Springer Science+Business Media, Inc., 2007 Bamboo nationallicence Cytokinin nationallicence TDZ nationallicence 2,4-D : 2,4-dichlorophenoxyacetic acid nationallicence BA : N6-benzyladenine nationallicence IBA : indolebutyric acid nationallicence 2-ip : 2-isopentyl adenine nationallicence MS : Murashige and Skoog medium nationallicence NAA : α-naphthaleneacetic acid nationallicence PVP : polyvinyl pyrrolidone nationallicence TDZ : thidiazuron nationallicence Lin Choun-Sea Institute of Plant and Microbiology, Academia Sinica, Taipei, Taiwan aut Liang C. Institute of Plant and Microbiology, Academia Sinica, Taipei, Taiwan aut Hsaio H. Department of Bioinformatics, Asia University, Taichung, Taiwan aut Lin M. Institute of Plant and Microbiology, Academia Sinica, Taipei, Taiwan aut Chang W. Institute of Plant and Microbiology, Academia Sinica, Taipei, Taiwan aut New Forests Kluwer Academic Publishers 34/2(2007-09-01), 177-186 0169-4286 34:2<177 2007 34 11056 https://doi.org/10.1007/s11056-007-9045-8 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11056-007-9045-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Lin Choun-Sea Institute of Plant and Microbiology, Academia Sinica, Taipei, Taiwan aut NATIONALLICENCE 700 1- Liang C. Institute of Plant and Microbiology, Academia Sinica, Taipei, Taiwan aut NATIONALLICENCE 700 1- Hsaio H. Department of Bioinformatics, Asia University, Taichung, Taiwan aut NATIONALLICENCE 700 1- Lin M. Institute of Plant and Microbiology, Academia Sinica, Taipei, Taiwan aut NATIONALLICENCE 700 1- Chang W. Institute of Plant and Microbiology, Academia Sinica, Taipei, Taiwan aut NATIONALLICENCE 773 0- New Forests Kluwer Academic Publishers 34/2(2007-09-01), 177-186 0169-4286 34:2<177 2007 34 11056 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer