caa a22 4500 463218256 CHVBK 20180405153204.0 cr unu---uuuuu 170326e20070901xx s 000 0 eng 10.1007/s11056-007-9048-5 doi (NATIONALLICENCE)springer-10.1007/s11056-007-9048-5 An assessment of heterozygosity and fitness in Chir pine ( Pinus roxburghii Sarg.) using isozymes [Elektronische Daten] [Kamal Sharma, Bernd Degen, Georg von Wuehlisch, Nar Singh] A correlation between heterozygosity of genotypes and survival (fitness) was studied in eight natural populations of Pinus roxburghii through isozyme analysis using horizontal starch gel electrophoresis. Under each population, 20 mother trees of different ages (30 to more than 100years) were selected which were at least 50m apart. From each tree, eight seeds were assayed for eleven enzyme systems viz., Aconitase, Aspertate aminotransferase, Glutamate dehydrogenase, Isocitrate dehydrogenase, Leucine-amino peptidase, Malate dehydrogenase, Menadione reductase, Phosphoglucose isomerase, Phosphoglucomutase, 6-Phosphogluconate dehydrogenase and Shikimic acid dehdrogenase which were found to be encoded by 18 polymorphic loci. Genetic constitution of the mother trees was determined by analysing endosperms and embryos separately. The number of alleles in the progeny (embryos) and the mother trees varied from 38 to 42 and 34 to 37, respectively, in all the populations. Distribution of degree of heterozygosity and fixation index for the progeny and the mother trees were calculated. The mother trees showed a substantial shift towards higher degree of heterozygosity as compared to the progeny. Fixation index values were significantly higher and negative for the mother trees as compared to the progeny, which revealed that heterozygosity was positively correlated with survival in P. roxburghii. Springer Science+Business Media, Inc., 2007 Pinus roxburghii nationallicence Allozymes nationallicence Multilocus diversity nationallicence Heterozygosity nationallicence Fixation index nationallicence Sharma Kamal Dr. YS Parmar University of Horticulture and Forestry, Regional Horticultural and Forestry Research Station, 176 041, District Hamirpur, Bhota, HP, India aut Degen Bernd Federal Research Centre for Forestry and Forest Products, Institute for Forest Genetics and Forest Tree Breeding, Sieker Landstr. 2, 22927, Grosshansdorf, Germany aut von Wuehlisch Georg Federal Research Centre for Forestry and Forest Products, Institute for Forest Genetics and Forest Tree Breeding, Sieker Landstr. 2, 22927, Grosshansdorf, Germany aut Singh Nar Department of Tree Improvement and Genetic Resources, Dr. YS Parmar University of Horticulture and Forestry, 173 230, Nauni (Solan), HP, India aut New Forests Kluwer Academic Publishers 34/2(2007-09-01), 153-162 0169-4286 34:2<153 2007 34 11056 https://doi.org/10.1007/s11056-007-9048-5 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11056-007-9048-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Sharma Kamal Dr. YS Parmar University of Horticulture and Forestry, Regional Horticultural and Forestry Research Station, 176 041, District Hamirpur, Bhota, HP, India aut NATIONALLICENCE 700 1- Degen Bernd Federal Research Centre for Forestry and Forest Products, Institute for Forest Genetics and Forest Tree Breeding, Sieker Landstr. 2, 22927, Grosshansdorf, Germany aut NATIONALLICENCE 700 1- von Wuehlisch Georg Federal Research Centre for Forestry and Forest Products, Institute for Forest Genetics and Forest Tree Breeding, Sieker Landstr. 2, 22927, Grosshansdorf, Germany aut NATIONALLICENCE 700 1- Singh Nar Department of Tree Improvement and Genetic Resources, Dr. YS Parmar University of Horticulture and Forestry, 173 230, Nauni (Solan), HP, India aut NATIONALLICENCE 773 0- New Forests Kluwer Academic Publishers 34/2(2007-09-01), 153-162 0169-4286 34:2<153 2007 34 11056 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer