caa a22 4500 463218590 CHVBK 20180405153205.0 cr unu---uuuuu 170326e20070101xx s 000 0 eng 10.1134/S1061934807010170 doi (NATIONALLICENCE)springer-10.1134/S1061934807010170 Chiral separation of ephedrine alkaloids from Ephedra sinica extract and its medicinal preparation using cyclodextrin-modified capillary zone electrophoresis [Elektronische Daten] [T. Jiang, Z. Lv, Y. Wang] A fast capillary zone electrophoresis (CZE) method using dimethyl-β-cyclodextrin (DM-β-CD) as modifier with tetrabutylammonium chloride (TBAC) as addition has been developed for the chiral separation of (±)-ephedrine ((±)-EP), (±)-pseudoephedrine ((±)-PP), (±)-N-methylephedrine ((±)-MP), and (±)-norephedrine ((±)-NP). The electrophoretic separation was performed using a 35-cm × 50 μm I.D. (30-cm effective length) fused silica capillary. Samples were introduced under electromigrated injection at 5 kV for 4 s, and the running voltage was 15 kV at the injector end of the capillary. Within 19 min, eight ephedrine compounds were separated and detected at 210 nm. The method was successively applied to the determination of the ephedrine enantiomers in the Chinese herbal extract from Ephedra sinica and its medicinal preparation (Xiaoerqing feiwan). Parameters affecting the resolution between (+) and (-)-enantiomers, such as pH, cyclodextrin type and concentration, organic modifier, and tetraalkylammonium reagents, were reported. Pleiades Publishing, Ltd., 2007 Jiang T. Key Laboratory of Marine Drugs (Ocean University of China), Ministry of Education, China aut Lv Z. Key Laboratory of Marine Drugs (Ocean University of China), Ministry of Education, China aut Wang Y. Key Laboratory of Marine Drugs (Ocean University of China), Ministry of Education, China aut Journal of Analytical Chemistry Nauka/Interperiodica 62/1(2007-01-01), 85-89 1061-9348 62:1<85 2007 62 10809 https://doi.org/10.1134/S1061934807010170 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1134/S1061934807010170 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Jiang T. Key Laboratory of Marine Drugs (Ocean University of China), Ministry of Education, China aut NATIONALLICENCE 700 1- Lv Z. Key Laboratory of Marine Drugs (Ocean University of China), Ministry of Education, China aut NATIONALLICENCE 700 1- Wang Y. Key Laboratory of Marine Drugs (Ocean University of China), Ministry of Education, China aut NATIONALLICENCE 773 0- Journal of Analytical Chemistry Nauka/Interperiodica 62/1(2007-01-01), 85-89 1061-9348 62:1<85 2007 62 10809 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer