caa a22 4500 463218604 CHVBK 20180405153205.0 cr unu---uuuuu 170326e20070101xx s 000 0 eng 10.1134/S106193480701011X doi (NATIONALLICENCE)springer-10.1134/S106193480701011X Determination of hydroxyproline in tissues and the evaluation of the collagen content of the tissues [Elektronische Daten] [N. Ignat'eva, N. Danilov, S. Averkiev, M. Obrezkova, V. Lunin, E. Sobol'] The concentrations of hydroxyproline (an amino acid specific of collagen) in a number of connective tissues were determined. Two procedures were compared. In one of them, amino acids were preseparated by chromatography and then determined on a standard amino acid analyzer. In the other procedure, hydroxyproline was selectively oxidized without amino acid separation and determined by a spectrophotometric reaction with Ehrlich's reagent. Data obtained for purified collagen preparations in accordance with the two procedures were consistent with each other. The results can be somewhat different in unpurified preparations and tissues because of the presence of polysaccharide components in the tissues. Pleiades Publishing, Ltd., 2007 Ignat'eva N. Department of Chemistry, Moscow State University, Vorob'evy gory 1, 119899, Moscow, Russia aut Danilov N. Department of Chemistry, Moscow State University, Vorob'evy gory 1, 119899, Moscow, Russia aut Averkiev S. Department of Chemistry, Moscow State University, Vorob'evy gory 1, 119899, Moscow, Russia aut Obrezkova M. Department of Chemistry, Moscow State University, Vorob'evy gory 1, 119899, Moscow, Russia aut Lunin V. Department of Chemistry, Moscow State University, Vorob'evy gory 1, 119899, Moscow, Russia aut Sobol' E. Institute of Laser and Information Technologies, Russian Academy of Sciences, 142092, Troitsk, Moscow oblast, Russia aut Journal of Analytical Chemistry Nauka/Interperiodica 62/1(2007-01-01), 51-57 1061-9348 62:1<51 2007 62 10809 https://doi.org/10.1134/S106193480701011X text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1134/S106193480701011X text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ignat'eva N. Department of Chemistry, Moscow State University, Vorob'evy gory 1, 119899, Moscow, Russia aut NATIONALLICENCE 700 1- Danilov N. Department of Chemistry, Moscow State University, Vorob'evy gory 1, 119899, Moscow, Russia aut NATIONALLICENCE 700 1- Averkiev S. Department of Chemistry, Moscow State University, Vorob'evy gory 1, 119899, Moscow, Russia aut NATIONALLICENCE 700 1- Obrezkova M. Department of Chemistry, Moscow State University, Vorob'evy gory 1, 119899, Moscow, Russia aut NATIONALLICENCE 700 1- Lunin V. Department of Chemistry, Moscow State University, Vorob'evy gory 1, 119899, Moscow, Russia aut NATIONALLICENCE 700 1- Sobol' E. Institute of Laser and Information Technologies, Russian Academy of Sciences, 142092, Troitsk, Moscow oblast, Russia aut NATIONALLICENCE 773 0- Journal of Analytical Chemistry Nauka/Interperiodica 62/1(2007-01-01), 51-57 1061-9348 62:1<51 2007 62 10809 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer