caa a22 4500 463219163 CHVBK 20180405153206.0 cr unu---uuuuu 170326e20070701xx s 000 0 eng 10.1134/S106193480707009X doi (NATIONALLICENCE)springer-10.1134/S106193480707009X Deineka V. Department of Biology and Chemistry, Belgorod State University, ul. Pobedy 85, 108015, Belgorod, Russia aut A new method for assessing the effect of residual silanol groups on total retention in reversed-phase HPLC [Elektronische Daten] [V. Deineka] A new approach is proposed to assess the effect of residual silanol groups (hydrophilicity) on the retention of polar substances in reversed-phase high-performance liquid chromatography (HPLC). It is shown that the numerical characteristic of the relative position of trend lines in the coordinates logk(B) vs. logk(A) of the relative retention parameters (at different mobile phase compositions) for any stationary phase relative to the stationary phase selected as a standard is preferable to the separation selectivity of the same pair of substances. Full information can be obtained by determining the retention of corresponding adsorbates with two different mobile phase compositions for each column under study. It was shown that the pair of adsorbates p-toluidine-p-cresol exhibits higher sensitivity to a change in the hydrophilicity of stationary phases as compared to the pair aniline-phenol. However, the use of a nonpolar substance as a reference compound is more informative. Pleiades Publishing, Ltd., 2007 Journal of Analytical Chemistry Nauka/Interperiodica 62/7(2007-07-01), 665-668 1061-9348 62:7<665 2007 62 10809 https://doi.org/10.1134/S106193480707009X text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1134/S106193480707009X text/html Onlinezugriff via DOI NATIONALLICENCE 100 1- Deineka V. Department of Biology and Chemistry, Belgorod State University, ul. Pobedy 85, 108015, Belgorod, Russia aut NATIONALLICENCE 773 0- Journal of Analytical Chemistry Nauka/Interperiodica 62/7(2007-07-01), 665-668 1061-9348 62:7<665 2007 62 10809 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer