caa a22 4500 463219309 CHVBK 20180405153207.0 cr unu---uuuuu 170326e20071201xx s 000 0 eng 10.1134/S1061934807120155 doi (NATIONALLICENCE)springer-10.1134/S1061934807120155 A potentiometric DNA sensor for determining autoimmune antibodies to DNA [Elektronische Daten] [A. Porfir'eva, G. Evtyugin, E. Podshivalina, L. Anchikova, G. Budnikov] A new technique is proposed for detecting interactions between DNA and DNA autoimmune antibodies using a potentiometric sensor based on a glassy-carbon electrode modified with polyaniline and native DNA from chicken erythrocytes. It is shown that the DNA-antibody interaction changes the rate of polyaniline doping in transferring the DNA sensor from an alkaline (pH 7.5) solution, which is optimum for the immunochemical reaction, to an acidic (pH 3.0) solution. The dynamics of the variation of the DNA sensor potential depends on the titer of antibodies and their origin. The dependence of the DNA sensor signal on the dilution of the blood sera from systemic lupus erythematosus and autoimmune thyroiditis patients shows that DNA antibodies can be diagnosed by the characteristic maximum in the dilution curve found in the range of serum dilutions from 1: 20 to 1: 50. Pleiades Publishing, Ltd., 2007 Porfir'eva A. Butlerov Institute of Chemistry, Kazan State University, ul. Kremlevskaya 18, 420008, Kazan, Tatarstan, Russia aut Evtyugin G. Butlerov Institute of Chemistry, Kazan State University, ul. Kremlevskaya 18, 420008, Kazan, Tatarstan, Russia aut Podshivalina E. Kazan State Medical Academy, ul. Mushtari 11, 420012, Kazan, Tatarstan, Russia aut Anchikova L. Kazan State Medical Academy, ul. Mushtari 11, 420012, Kazan, Tatarstan, Russia aut Budnikov G. Butlerov Institute of Chemistry, Kazan State University, ul. Kremlevskaya 18, 420008, Kazan, Tatarstan, Russia aut Journal of Analytical Chemistry Nauka/Interperiodica 62/12(2007-12-01), 1180-1186 1061-9348 62:12<1180 2007 62 10809 https://doi.org/10.1134/S1061934807120155 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1134/S1061934807120155 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Porfir'eva A. Butlerov Institute of Chemistry, Kazan State University, ul. Kremlevskaya 18, 420008, Kazan, Tatarstan, Russia aut NATIONALLICENCE 700 1- Evtyugin G. Butlerov Institute of Chemistry, Kazan State University, ul. Kremlevskaya 18, 420008, Kazan, Tatarstan, Russia aut NATIONALLICENCE 700 1- Podshivalina E. Kazan State Medical Academy, ul. Mushtari 11, 420012, Kazan, Tatarstan, Russia aut NATIONALLICENCE 700 1- Anchikova L. Kazan State Medical Academy, ul. Mushtari 11, 420012, Kazan, Tatarstan, Russia aut NATIONALLICENCE 700 1- Budnikov G. Butlerov Institute of Chemistry, Kazan State University, ul. Kremlevskaya 18, 420008, Kazan, Tatarstan, Russia aut NATIONALLICENCE 773 0- Journal of Analytical Chemistry Nauka/Interperiodica 62/12(2007-12-01), 1180-1186 1061-9348 62:12<1180 2007 62 10809 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer