caa a22 4500 463219775 CHVBK 20180405153208.0 cr unu---uuuuu 170326e20071001xx s 000 0 eng 10.1134/S1061934807100097 doi (NATIONALLICENCE)springer-10.1134/S1061934807100097 Chromatofocusing of peptides on a sulfo-cation-exchange sorbent [Elektronische Daten] [M. Vakshtein, A. Ivanov] Simple mobile phases containing no more than two active components were proposed for the formation of ascending pH gradients in a column filled with a sulfo-cation-exchange sorbent. The smoothest nearly linear pH gradients were obtained with the use of citric acid and Tris or NaH2PO4 and Tris as active components of the eluent and adjusting ionic strength (up to 0.1-0.3) in the starting solution or eluent; however, in the case of UV detection, the use of NaH2PO4 is preferable because of lower light absorption. Potentialities of the proposed approach in the chromatography of peptide mixtures on a sulfo-cation-exchange sorbent were demonstrated. Additions of acetonitrile to mobile phases improve the selectivity of the separation of peptides. Pleiades Publishing, Ltd., 2007 Vakshtein M. Department of Chemistry, Moscow State University, 119992, Vorob'evy gory, Moscow, Russia aut Ivanov A. Department of Chemistry, Moscow State University, 119992, Vorob'evy gory, Moscow, Russia aut Journal of Analytical Chemistry Nauka/Interperiodica 62/10(2007-10-01), 951-956 1061-9348 62:10<951 2007 62 10809 https://doi.org/10.1134/S1061934807100097 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1134/S1061934807100097 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Vakshtein M. Department of Chemistry, Moscow State University, 119992, Vorob'evy gory, Moscow, Russia aut NATIONALLICENCE 700 1- Ivanov A. Department of Chemistry, Moscow State University, 119992, Vorob'evy gory, Moscow, Russia aut NATIONALLICENCE 773 0- Journal of Analytical Chemistry Nauka/Interperiodica 62/10(2007-10-01), 951-956 1061-9348 62:10<951 2007 62 10809 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer