caa a22 4500 463219872 CHVBK 20180405153208.0 cr unu---uuuuu 170326e20070401xx s 000 0 eng 10.1134/S1061934807040132 doi (NATIONALLICENCE)springer-10.1134/S1061934807040132 HPLC analysis of mitochondrial membrane phospholipids in rice [Elektronische Daten] [F. He, C. Wang, X. Li, X. Liu] The effects of different columns and mobile phases on the separation of mitochondrial membrane phospholipids in rice by high-performance liquid chromatography (HPLC) were studied. The results suggest that the main six kinds of phospholipids in the rice mitochondrial membrane can be successfully separated within 22 min on a KR 100-SIL (250 × 4.6 mm, 5 μm) column with a mobile phase of acetonitrile-methanol-85% phosphoric acid (100: 10: 0.8, V/V) and DAD detector (205 nm). The quantitative analysis of five important components—phospatidylinositol (PI), phosphatidylethanolamine (PE), phosphatidylserine (PS), phosphotidic acid (PA), and phosphatidylcloline (PC)—has been achieved with an external standard method. The linear range is 0.005-2.00 mg/mL, the recovery ratio is 95.9-100.6%, and the relative standard deviation is 0.32-1.24%. The study indicates that the HPLC method that has been applied to the analysis of the trace content of biomembrane phospholipids is characterized by a satisfactory linear relationship, reproducibility, and stability. Pleiades Publishing, Ltd., 2007 He F. Key Laboratory for Biotechnology of State Ethnic Affairs Commission, College of Life Sciences, South Central University for Nationalities, 430074, Wuhan, China aut Wang C. Key Laboratory for Biotechnology of State Ethnic Affairs Commission, College of Life Sciences, South Central University for Nationalities, 430074, Wuhan, China aut Li X. Key Laboratory for Biotechnology of State Ethnic Affairs Commission, College of Life Sciences, South Central University for Nationalities, 430074, Wuhan, China aut Liu X. Key Laboratory for Biotechnology of State Ethnic Affairs Commission, College of Life Sciences, South Central University for Nationalities, 430074, Wuhan, China aut Journal of Analytical Chemistry Nauka/Interperiodica 62/4(2007-04-01), 369-372 1061-9348 62:4<369 2007 62 10809 https://doi.org/10.1134/S1061934807040132 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1134/S1061934807040132 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- He F. Key Laboratory for Biotechnology of State Ethnic Affairs Commission, College of Life Sciences, South Central University for Nationalities, 430074, Wuhan, China aut NATIONALLICENCE 700 1- Wang C. Key Laboratory for Biotechnology of State Ethnic Affairs Commission, College of Life Sciences, South Central University for Nationalities, 430074, Wuhan, China aut NATIONALLICENCE 700 1- Li X. Key Laboratory for Biotechnology of State Ethnic Affairs Commission, College of Life Sciences, South Central University for Nationalities, 430074, Wuhan, China aut NATIONALLICENCE 700 1- Liu X. Key Laboratory for Biotechnology of State Ethnic Affairs Commission, College of Life Sciences, South Central University for Nationalities, 430074, Wuhan, China aut NATIONALLICENCE 773 0- Journal of Analytical Chemistry Nauka/Interperiodica 62/4(2007-04-01), 369-372 1061-9348 62:4<369 2007 62 10809 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer