caa a22 4500 463220226 CHVBK 20180405153209.0 cr unu---uuuuu 170326e20070501xx s 000 0 eng 10.1134/S1061934807050085 doi (NATIONALLICENCE)springer-10.1134/S1061934807050085 Study of a fluorescence quenching mechanism of enoxacin and its determination in human serum and urine samples [Elektronische Daten] [Yan Xiao, Jian Wang, Xia Feng, Huai Wang] The maximum emission wavelength of dopamine is 317 nm with excitation at 290 nm. The relative fluorescence intensity of dopamine decreased in the presence of enoxacin, which showed that fluorescence quenching occurred. The Stern-Volmer (S-V) plot showed a nonlinear relationship between the relative fluorescence intensity of dopamine and the concentration of enoxain. The quenching mechanism was studied and the results suggested that both dynamic and static quenching processes were responsible for the observed positive deviation in the S-V plot. When the S-V plot was modified by logarithm, the linear relationship was obtained between logF 0/F and C in the range of 0.10 to 13.0 μg/mL (where F 0 is the relative fluorescence intensity of dopamine, F is the relative fluorescence intensity of dopamine in the presence of enoxacin, and C is the concentration of enoxacin). The fluorescence quenching method for the determination of enoxacin was developed. The linear regression equation of the calibration graph of enoxacin was C = 13.70 (logF 0/F) − 0.5836, with the correlation coefficient 0.9984. The detection limit was 2.0 ng/mL and the relative standard deviation was 2.52%. The effects of pH, the stability of dopamine in the presence of enoxacin, and foreign ions on the determination of enoxacin have been examined. The recovery of enoxacin was from 94.9 to 103.0% in a human serum sample and from 94.9 to 108.0% in a urine sample. The method is simple, rapid, and can be used for the determination of enoxacin in human serum and urine samples with satisfactory results. Pleiades Publishing, Ltd., 2007 Xiao Yan College of Chemistry, Chemical Engineering and Materials Science, Shandong Normal University, 250014, Jinan, China aut Wang Jian Zaozhuang Occupational Technology College, 277100, Zaozhuang, Shandong Province, China aut Feng Xia College of Chemistry, Chemical Engineering and Materials Science, Shandong Normal University, 250014, Jinan, China aut Wang Huai College of Chemistry, Chemical Engineering and Materials Science, Shandong Normal University, 250014, Jinan, China aut Journal of Analytical Chemistry Nauka/Interperiodica 62/5(2007-05-01), 438-443 1061-9348 62:5<438 2007 62 10809 https://doi.org/10.1134/S1061934807050085 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1134/S1061934807050085 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Xiao Yan College of Chemistry, Chemical Engineering and Materials Science, Shandong Normal University, 250014, Jinan, China aut NATIONALLICENCE 700 1- Wang Jian Zaozhuang Occupational Technology College, 277100, Zaozhuang, Shandong Province, China aut NATIONALLICENCE 700 1- Feng Xia College of Chemistry, Chemical Engineering and Materials Science, Shandong Normal University, 250014, Jinan, China aut NATIONALLICENCE 700 1- Wang Huai College of Chemistry, Chemical Engineering and Materials Science, Shandong Normal University, 250014, Jinan, China aut NATIONALLICENCE 773 0- Journal of Analytical Chemistry Nauka/Interperiodica 62/5(2007-05-01), 438-443 1061-9348 62:5<438 2007 62 10809 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer