caa a22 4500 463220374 CHVBK 20180405153210.0 cr unu---uuuuu 170326e20070501xx s 000 0 eng 10.1134/S1061934807050061 doi (NATIONALLICENCE)springer-10.1134/S1061934807050061 Spectrophotometric determination of hemoglobin with p -acetylarsenazo [Elektronische Daten] [Q. Zhai, J. Li, T. Chen] In a Britton-Robinson buffer medium at pH 2.87, hemoglobin can form a color compound with p-acetylarsenazo in the presence of emulgent OP. The maximum absorption locates at 370 nm. The apparent molar absorptivity ɛ370 nm is 4.11 × 105 L/(mol cm). Beer's law is obeyed over the range of 20-60 mg/L for hemoglobin. After the addition of a suitable amount of emulgent OP, the sensitivity and stability of the system can be obviously enhanced. Ethanol can increase the sensitivity of the system. Sodium chloride can enhance the sensitivity of the color system by 30%. The method has been applied to the determination of total proteins in human serums with satisfactory results. Pleiades Publishing, Ltd., 2007 Zhai Q. Research Center for Nanotechnology, Changchun University of Science and Technology, Changchun, 7186 Weixing Road, 130022, P.R. China aut Li J. Research Center for Nanotechnology, Changchun University of Science and Technology, Changchun, 7186 Weixing Road, 130022, P.R. China aut Chen T. Research Center for Nanotechnology, Changchun University of Science and Technology, Changchun, 7186 Weixing Road, 130022, P.R. China aut Journal of Analytical Chemistry Nauka/Interperiodica 62/5(2007-05-01), 428-431 1061-9348 62:5<428 2007 62 10809 https://doi.org/10.1134/S1061934807050061 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1134/S1061934807050061 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zhai Q. Research Center for Nanotechnology, Changchun University of Science and Technology, Changchun, 7186 Weixing Road, 130022, P.R. China aut NATIONALLICENCE 700 1- Li J. Research Center for Nanotechnology, Changchun University of Science and Technology, Changchun, 7186 Weixing Road, 130022, P.R. China aut NATIONALLICENCE 700 1- Chen T. Research Center for Nanotechnology, Changchun University of Science and Technology, Changchun, 7186 Weixing Road, 130022, P.R. China aut NATIONALLICENCE 773 0- Journal of Analytical Chemistry Nauka/Interperiodica 62/5(2007-05-01), 428-431 1061-9348 62:5<428 2007 62 10809 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer