caa a22 4500 463220676 CHVBK 20180405153211.0 cr unu---uuuuu 170326e20071101xx s 000 0 eng 10.1134/S106193480711010X doi (NATIONALLICENCE)springer-10.1134/S106193480711010X Determination of α-tocopherol and ergocalciferol by thin-layer chromatography [Elektronische Daten] [E. Borodina, T. Kitaeva, E. Safonova, V. Selemenev, A. Nazarova] A procedure was developed for determining α-tocopherol (vitamin E) and ergocalciferol (vitamin D2) in oils. The procedure involved the alkaline hydrolysis of an oil according to GOST (State Standard) 30417 followed by the independent recovery of vitamins by extraction with organic solvents. The extracts were separated on Sorbfil plates with the stationary phase of silica gel with a particle size of 5-12 μm by eluting vitamin E with an octane-diethyl ether mixture (7: 1) and vitamin D2 with a tetrachloromethane-diethyl ether mixture (4: 1). The zones of α-tocopherol and ergocalciferol were developed with conc. HNO3 and conc. H2SO4, respectively. The plate scanning and image processing were performed with a Sorbfil Videodensitometer software. The calibration plots were linear in the range of 2.5-15 and 15-50 mg/mL for α-tocopherol and 0.08-0.13 mg/mL for ergocalciferol. The procedure was used to determine vitamins in bur marigold oily extract and grape seed oil. Pleiades Publishing, Ltd., 2007 Borodina E. Voronezh State University, Universitetskaya pl. 1, 394693, Voronezh, Russia aut Kitaeva T. Voronezh State University, Universitetskaya pl. 1, 394693, Voronezh, Russia aut Safonova E. Voronezh State University, Universitetskaya pl. 1, 394693, Voronezh, Russia aut Selemenev V. Voronezh State University, Universitetskaya pl. 1, 394693, Voronezh, Russia aut Nazarova A. Voronezh State University, Universitetskaya pl. 1, 394693, Voronezh, Russia aut Journal of Analytical Chemistry Nauka/Interperiodica 62/11(2007-11-01), 1064-1068 1061-9348 62:11<1064 2007 62 10809 https://doi.org/10.1134/S106193480711010X text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1134/S106193480711010X text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Borodina E. Voronezh State University, Universitetskaya pl. 1, 394693, Voronezh, Russia aut NATIONALLICENCE 700 1- Kitaeva T. Voronezh State University, Universitetskaya pl. 1, 394693, Voronezh, Russia aut NATIONALLICENCE 700 1- Safonova E. Voronezh State University, Universitetskaya pl. 1, 394693, Voronezh, Russia aut NATIONALLICENCE 700 1- Selemenev V. Voronezh State University, Universitetskaya pl. 1, 394693, Voronezh, Russia aut NATIONALLICENCE 700 1- Nazarova A. Voronezh State University, Universitetskaya pl. 1, 394693, Voronezh, Russia aut NATIONALLICENCE 773 0- Journal of Analytical Chemistry Nauka/Interperiodica 62/11(2007-11-01), 1064-1068 1061-9348 62:11<1064 2007 62 10809 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer