caa a22 4500 463224132 CHVBK 20180405153221.0 cr unu---uuuuu 170326e20070501xx s 000 0 eng 10.1007/s10853-006-0235-0 doi (NATIONALLICENCE)springer-10.1007/s10853-006-0235-0 Immobilization of purine bases on a poly-4-aminophenol matrix [Elektronische Daten] [Ana Brito-Madurro, Lucas Ferreira, Sabrina Vieira, Rafael Ariza, Luiz Filho, João Madurro] Conducting polymers or semi-conductors have various features that make them excellent materials for the immobilization of biomolecules and the rapid transfer of electrons necessary for the production of biosensors. Conducting electroactive polymers of poly-4-aminophenol have been developed as sensors to detect the purine bases (adenosine triphosphate, ATP and guanosine triphosphate, GTP) of DNA. The electrooxidation of 4-aminophenol onto a graphite electrode in the presence of perchloric acid yielded thin polymer films. The conductivity was studied by cyclic voltammetry and surface morphology by optical microscopy and interferometry. The immobilization and detection of ATP and GTP on a graphite electrode or modified electrode coated with poly-4-aminophenol was studied by cyclic voltammetry. Systematic variation of the experimental conditions that influenced the electrode reaction, particularly the pH of the electrolytic solution, showed that the oxidation potentials of the immobilized ATP or GTP in the modified electrodes decreased with increasing pH of the electrolyte. When these conditions were optimized based on voltammetric measurements, modified electrodes coated with poly-4-aminophenol were found to be efficient in immobilizing purine bases, and increased the amplitude of the ATP and GTP signals by ∼1.5 and ∼24 times, respectively, when compared with non-coated graphite surfaces. Springer Science+Business Media, LLC, 2007 Brito-Madurro Ana Instituto de Química, Laboratório de Filmes Poliméricos, Universidade Federal de Uberlândia (UFU), Avenida João Naves de Ávila, 2121, Caixa Postal 593, 38400-902, Uberlandia, MG, Brazil aut Ferreira Lucas Instituto de Química, Laboratório de Filmes Poliméricos, Universidade Federal de Uberlândia (UFU), Avenida João Naves de Ávila, 2121, Caixa Postal 593, 38400-902, Uberlandia, MG, Brazil aut Vieira Sabrina Instituto de Química, Laboratório de Filmes Poliméricos, Universidade Federal de Uberlândia (UFU), Avenida João Naves de Ávila, 2121, Caixa Postal 593, 38400-902, Uberlandia, MG, Brazil aut Ariza Rafael Faculdade de Engenharia Mecânica, Laboratório de Tribologia de Materiais, Universidade Federal de Uberlândia (UFU), 38400-902, Uberlandia, MG, Brazil aut Filho Luiz Instituto de Genética e Bioquímica, Laboratório de Genética Molecular, Universidade Federal de Uberlândia (UFU), 38400-902, Uberlandia, MG, Brazil aut Madurro João Instituto de Química, Laboratório de Filmes Poliméricos, Universidade Federal de Uberlândia (UFU), Avenida João Naves de Ávila, 2121, Caixa Postal 593, 38400-902, Uberlandia, MG, Brazil aut Journal of Materials Science Kluwer Academic Publishers-Plenum Publishers; http://www.springer-ny.com 42/9(2007-05-01), 3238-3243 0022-2461 42:9<3238 2007 42 10853 https://doi.org/10.1007/s10853-006-0235-0 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10853-006-0235-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Brito-Madurro Ana Instituto de Química, Laboratório de Filmes Poliméricos, Universidade Federal de Uberlândia (UFU), Avenida João Naves de Ávila, 2121, Caixa Postal 593, 38400-902, Uberlandia, MG, Brazil aut NATIONALLICENCE 700 1- Ferreira Lucas Instituto de Química, Laboratório de Filmes Poliméricos, Universidade Federal de Uberlândia (UFU), Avenida João Naves de Ávila, 2121, Caixa Postal 593, 38400-902, Uberlandia, MG, Brazil aut NATIONALLICENCE 700 1- Vieira Sabrina Instituto de Química, Laboratório de Filmes Poliméricos, Universidade Federal de Uberlândia (UFU), Avenida João Naves de Ávila, 2121, Caixa Postal 593, 38400-902, Uberlandia, MG, Brazil aut NATIONALLICENCE 700 1- Ariza Rafael Faculdade de Engenharia Mecânica, Laboratório de Tribologia de Materiais, Universidade Federal de Uberlândia (UFU), 38400-902, Uberlandia, MG, Brazil aut NATIONALLICENCE 700 1- Filho Luiz Instituto de Genética e Bioquímica, Laboratório de Genética Molecular, Universidade Federal de Uberlândia (UFU), 38400-902, Uberlandia, MG, Brazil aut NATIONALLICENCE 700 1- Madurro João Instituto de Química, Laboratório de Filmes Poliméricos, Universidade Federal de Uberlândia (UFU), Avenida João Naves de Ávila, 2121, Caixa Postal 593, 38400-902, Uberlandia, MG, Brazil aut NATIONALLICENCE 773 0- Journal of Materials Science Kluwer Academic Publishers-Plenum Publishers; http://www.springer-ny.com 42/9(2007-05-01), 3238-3243 0022-2461 42:9<3238 2007 42 10853 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer