caa a22 4500 463225856 CHVBK 20180405153226.0 cr unu---uuuuu 170326e20071101xx s 000 0 eng 10.1007/s10853-007-1809-1 doi (NATIONALLICENCE)springer-10.1007/s10853-007-1809-1 Oriented immobilization of IgG on hydroxylated Si(001) surfaces via protein-A by a multiple-step process based on a self-assembly approach [Elektronische Daten] [G. Demirel, M. Çağlayan, B. Garipcan, M. Duman, E. Pişkin] The aim of this study was the oriented immobilization of IgG molecules on the silicon surfaces. A multiple-step procedure was applied for oriented immobilization of IgG in this study. After hydroxylation of the Si(001) surfaces, 3-glycidoxypropyltrimethoxysilane (GPTS) molecules were self-assembled onto these substrates. Dipping time and GPTS concentration were found to be effected by on both layer thicknesses and water-contact angles. 2,2′-(ethylenedioxy)diethylamine (EDA) molecules were then covalently attached to the silicon surface with GPTS molecules. There was no effect of concentration on the formation of EDA molecules on the surfaces, while EDA deposition increased with the dipping time significantly. Imaging ellipsometry and atomic force microscopy (AFM) images exhibited aggregate formation at this step. Protein-A molecules were bound to the free amino groups of EDA molecules on the substrate surface, especially onto the aggregates by using a carbodiimide (i.e., EDAC) as the activating agent. We were able to immobilize IgG molecules in an oriented form onto the protein-A attached surfaces, especially in the regions, where EDA aggregates are located. Springer Science+Business Media, LLC, 2007 Demirel G. Department of Chemical Engineering and Division of Bioengineering, Hacettepe University, Beytepe, 06800, Ankara, Turkey aut Çağlayan M. Department of Chemical Engineering and Division of Bioengineering, Hacettepe University, Beytepe, 06800, Ankara, Turkey aut Garipcan B. Department of Chemical Engineering and Division of Bioengineering, Hacettepe University, Beytepe, 06800, Ankara, Turkey aut Duman M. Department of Chemical Engineering and Division of Bioengineering, Hacettepe University, Beytepe, 06800, Ankara, Turkey aut Pişkin E. Department of Chemical Engineering and Division of Bioengineering, Hacettepe University, Beytepe, 06800, Ankara, Turkey aut Journal of Materials Science Springer US; http://www.springer-ny.com 42/22(2007-11-01), 9402-9408 0022-2461 42:22<9402 2007 42 10853 https://doi.org/10.1007/s10853-007-1809-1 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10853-007-1809-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Demirel G. Department of Chemical Engineering and Division of Bioengineering, Hacettepe University, Beytepe, 06800, Ankara, Turkey aut NATIONALLICENCE 700 1- Çağlayan M. Department of Chemical Engineering and Division of Bioengineering, Hacettepe University, Beytepe, 06800, Ankara, Turkey aut NATIONALLICENCE 700 1- Garipcan B. Department of Chemical Engineering and Division of Bioengineering, Hacettepe University, Beytepe, 06800, Ankara, Turkey aut NATIONALLICENCE 700 1- Duman M. Department of Chemical Engineering and Division of Bioengineering, Hacettepe University, Beytepe, 06800, Ankara, Turkey aut NATIONALLICENCE 700 1- Pişkin E. Department of Chemical Engineering and Division of Bioengineering, Hacettepe University, Beytepe, 06800, Ankara, Turkey aut NATIONALLICENCE 773 0- Journal of Materials Science Springer US; http://www.springer-ny.com 42/22(2007-11-01), 9402-9408 0022-2461 42:22<9402 2007 42 10853 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer