caa a22 4500 463234847 CHVBK 20180405153253.0 cr unu---uuuuu 170326e20070201xx s 000 0 eng 10.1007/s10404-006-0091-x doi (NATIONALLICENCE)springer-10.1007/s10404-006-0091-x Evaluation of micromilled metal mold masters for the replication of microchip electrophoresis devices [Elektronische Daten] [Mateusz Hupert, W. Guy, Shawn Llopis, Hamed Shadpour, Sudheer Rani, Dimitris Nikitopoulos, Steven Soper] High-precision micromilling was assessed as a tool for the rapid fabrication of mold masters for replicating microchip devices in thermoplastics. As an example, microchip electrophoresis devices were hot embossed in poly(methylmethacrylate) (PMMA) from brass masters fabricated via micromilling. Specifically, sidewall roughness and milling topology limitations were investigated. Numerical simulations were performed to determine the effects of additional volumes present on injection plugs (i.e., shape, size, concentration profiles) due to curvature of the corners produced by micromilling. Elongation of the plug was not dramatic (<20%) for injection crosses with radii of curvatures to channel width ratios less than 0.5. Use of stronger pinching potentials, as compared to sharp-corner injectors, were necessary in order to obtain short sample plugs. The sidewalls of the polymer microstructures were characterized by a maximum average roughness of 115nm and mean peak height of 290nm. Sidewall roughness had insignificant effects on the bulk EOF as it was statistically the same for PMMA microchannels with different aspect ratios compared to LiGA-prepared devices with a value of ca. 3.7×10−4cm2/(Vs). PMMA microchip electrophoresis devices were used for the separation of pUC19 Sau3AI double-stranded DNA. The plate numbers achieved in the micromilled-based chips exceeded 1million/m and were comparable to the plate numbers obtained for the LiGA-prepared devices of similar geometry. Springer-Verlag, 2006 Micromilling nationallicence Hot-embossing nationallicence Microchip electrophoresis nationallicence Polymer microfluidics nationallicence Hupert Mateusz Center for Bio-Modular Multi-Scale Systems, Louisiana State University, 8000 G.S.R.I. Rd., Blg. 3100, 70820-7403, Baton Rouge, LA, USA aut Guy W. Center for Bio-Modular Multi-Scale Systems, Louisiana State University, 8000 G.S.R.I. Rd., Blg. 3100, 70820-7403, Baton Rouge, LA, USA aut Llopis Shawn Center for Bio-Modular Multi-Scale Systems, Louisiana State University, 8000 G.S.R.I. Rd., Blg. 3100, 70820-7403, Baton Rouge, LA, USA aut Shadpour Hamed Center for Bio-Modular Multi-Scale Systems, Louisiana State University, 8000 G.S.R.I. Rd., Blg. 3100, 70820-7403, Baton Rouge, LA, USA aut Rani Sudheer Center for Bio-Modular Multi-Scale Systems, Louisiana State University, 8000 G.S.R.I. Rd., Blg. 3100, 70820-7403, Baton Rouge, LA, USA aut Nikitopoulos Dimitris Center for Bio-Modular Multi-Scale Systems, Louisiana State University, 8000 G.S.R.I. Rd., Blg. 3100, 70820-7403, Baton Rouge, LA, USA aut Soper Steven Center for Bio-Modular Multi-Scale Systems, Louisiana State University, 8000 G.S.R.I. Rd., Blg. 3100, 70820-7403, Baton Rouge, LA, USA aut Microfluidics and Nanofluidics Springer-Verlag 3/1(2007-02-01), 1-11 1613-4982 3:1<1 2007 3 10404 https://doi.org/10.1007/s10404-006-0091-x text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10404-006-0091-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Hupert Mateusz Center for Bio-Modular Multi-Scale Systems, Louisiana State University, 8000 G.S.R.I. Rd., Blg. 3100, 70820-7403, Baton Rouge, LA, USA aut NATIONALLICENCE 700 1- Guy W. Center for Bio-Modular Multi-Scale Systems, Louisiana State University, 8000 G.S.R.I. Rd., Blg. 3100, 70820-7403, Baton Rouge, LA, USA aut NATIONALLICENCE 700 1- Llopis Shawn Center for Bio-Modular Multi-Scale Systems, Louisiana State University, 8000 G.S.R.I. Rd., Blg. 3100, 70820-7403, Baton Rouge, LA, USA aut NATIONALLICENCE 700 1- Shadpour Hamed Center for Bio-Modular Multi-Scale Systems, Louisiana State University, 8000 G.S.R.I. Rd., Blg. 3100, 70820-7403, Baton Rouge, LA, USA aut NATIONALLICENCE 700 1- Rani Sudheer Center for Bio-Modular Multi-Scale Systems, Louisiana State University, 8000 G.S.R.I. Rd., Blg. 3100, 70820-7403, Baton Rouge, LA, USA aut NATIONALLICENCE 700 1- Nikitopoulos Dimitris Center for Bio-Modular Multi-Scale Systems, Louisiana State University, 8000 G.S.R.I. Rd., Blg. 3100, 70820-7403, Baton Rouge, LA, USA aut NATIONALLICENCE 700 1- Soper Steven Center for Bio-Modular Multi-Scale Systems, Louisiana State University, 8000 G.S.R.I. Rd., Blg. 3100, 70820-7403, Baton Rouge, LA, USA aut NATIONALLICENCE 773 0- Microfluidics and Nanofluidics Springer-Verlag 3/1(2007-02-01), 1-11 1613-4982 3:1<1 2007 3 10404 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer