caa a22 4500 46323507X CHVBK 20180405153254.0 cr unu---uuuuu 170326e20071201xx s 000 0 eng 10.1007/s10404-007-0152-9 doi (NATIONALLICENCE)springer-10.1007/s10404-007-0152-9 Combining fluidic reservoirs and optical tweezers to control beads/living cells contacts [Elektronische Daten] [Serge Monneret, Federico Belloni, Olivier Soppera] We have developed a complete system based on holographic optical tweezers to realize multiple-point interactions between beads and cells with control of the stimulation places, timing and durations. We introduce microstereolithography as a 3D micromanufacturing approach to the rapid prototyping of three-dimensional fluidic microchambers of complex shapes, comprising wells, channels and walls, that are afterwards placed inside the sample and used to inject beads locally and keep them separated from cells in our assays. A custom reservoir designed to keep beads and cells separated in liquid samples has been realized and successfully tested. This allows us to deposit beads locally on the microscope cover glass placed under the reservoir outlet. Limited dispersion of beads under the outlet has been confirmed, and the ability of the polymeric structures to confine beads in a restricted area has been demonstrated. Examples of manipulations consisting at first in extracting several beads from the reservoir, making them travel to the target cell, and finally depositing on its outer membrane with respect to the shape of the target cell, are finally given. Springer-Verlag, 2007 Optical tweezers nationallicence Microstereolithography nationallicence Microfluidics nationallicence Cell activation nationallicence Monneret Serge Mosaic group, Institut Fresnel, CNRS - Université Paul Cézanne, Marseille, France aut Belloni Federico Mosaic group, Institut Fresnel, CNRS - Université Paul Cézanne, Marseille, France aut Soppera Olivier Département de Photochimie Générale, CNRS - ENSC Mulhouse, Mulhouse, France aut Microfluidics and Nanofluidics Springer-Verlag 3/6(2007-12-01), 645-652 1613-4982 3:6<645 2007 3 10404 https://doi.org/10.1007/s10404-007-0152-9 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10404-007-0152-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Monneret Serge Mosaic group, Institut Fresnel, CNRS - Université Paul Cézanne, Marseille, France aut NATIONALLICENCE 700 1- Belloni Federico Mosaic group, Institut Fresnel, CNRS - Université Paul Cézanne, Marseille, France aut NATIONALLICENCE 700 1- Soppera Olivier Département de Photochimie Générale, CNRS - ENSC Mulhouse, Mulhouse, France aut NATIONALLICENCE 773 0- Microfluidics and Nanofluidics Springer-Verlag 3/6(2007-12-01), 645-652 1613-4982 3:6<645 2007 3 10404 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer