caa a22 4500 463235118 CHVBK 20180405153254.0 cr unu---uuuuu 170326e20070601xx s 000 0 eng 10.1007/s10404-006-0123-6 doi (NATIONALLICENCE)springer-10.1007/s10404-006-0123-6 System-level modeling and simulation of biochemical assays in lab-on-a-chip devices [Elektronische Daten] [Yi Wang, Aditya Bedekar, S. Krishnamoorthy, Sachin Siddhaye, Shivshankar Sundaram] We present a "mixed-methodology” based system-level modeling and simulation for biochemical assays in lab-on-a-chip (LoC) devices. The methodology uses a combination of numerical schemes and analytical approaches to simulate biological and physicochemical processes, specifically, an integral approach for fluid flow and electric field, method of lines (MOL) and two-compartment models for biochemical reactions, and Fourier series-based model for analyte mixing. The solution procedure begins with decomposing the LoC device into a system of inter-connected components (e.g., channels and junctions) and the models are solved in a network fashion. Models are developed to accurately capture the multi-physics (e.g., flow, mixing, and reaction) behavior of individual components. The assembly of the components is facilitated via exchange of fluid flux and Fourier series coefficients (or average concentration) of analytes between various components, which enables network solution of the models. The system models are validated against both experimental and numerical models on various biochemical assays (e.g. immunoassays and enzymatic reactions), showing significant computational speedup (100-10,000-fold depending on the assay) without appreciably compromising accuracy (<10% error relative to numerical analysis). Springer-Verlag, 2006 Modeling nationallicence Simulation nationallicence Lab-on-a-chip nationallicence Biochemical assay nationallicence Wang Yi CFD Research Corporation, 215 Wynn Drive, 35805, Huntsville, AL, USA aut Bedekar Aditya CFD Research Corporation, 215 Wynn Drive, 35805, Huntsville, AL, USA aut Krishnamoorthy S. CFD Research Corporation, 215 Wynn Drive, 35805, Huntsville, AL, USA aut Siddhaye Sachin CFD Research Corporation, 215 Wynn Drive, 35805, Huntsville, AL, USA aut Sundaram Shivshankar CFD Research Corporation, 215 Wynn Drive, 35805, Huntsville, AL, USA aut Microfluidics and Nanofluidics Springer-Verlag 3/3(2007-06-01), 307-322 1613-4982 3:3<307 2007 3 10404 https://doi.org/10.1007/s10404-006-0123-6 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10404-006-0123-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Wang Yi CFD Research Corporation, 215 Wynn Drive, 35805, Huntsville, AL, USA aut NATIONALLICENCE 700 1- Bedekar Aditya CFD Research Corporation, 215 Wynn Drive, 35805, Huntsville, AL, USA aut NATIONALLICENCE 700 1- Krishnamoorthy S. CFD Research Corporation, 215 Wynn Drive, 35805, Huntsville, AL, USA aut NATIONALLICENCE 700 1- Siddhaye Sachin CFD Research Corporation, 215 Wynn Drive, 35805, Huntsville, AL, USA aut NATIONALLICENCE 700 1- Sundaram Shivshankar CFD Research Corporation, 215 Wynn Drive, 35805, Huntsville, AL, USA aut NATIONALLICENCE 773 0- Microfluidics and Nanofluidics Springer-Verlag 3/3(2007-06-01), 307-322 1613-4982 3:3<307 2007 3 10404 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer