caa a22 4500 463235126 CHVBK 20180405153254.0 cr unu---uuuuu 170326e20070601xx s 000 0 eng 10.1007/s10404-006-0126-3 doi (NATIONALLICENCE)springer-10.1007/s10404-006-0126-3 Modeling and experimental characterization of peak tailing in DNA gel electrophoresis [Elektronische Daten] [Yi Sun, Yien Kwok, Nam-Trung Nguyen] Capillary electrophoresis (CE) is an efficient separation method in analytical chemistry. It exploits the difference in electrophoretic migration velocities between charged molecular species in aqueous or diluted polymer solution when an external electric field is applied to achieve separation. Despite the standard assumption that electrophoretic data obtained from pulse-loaded molecular species should have Gaussian peak shapes, experimentally observed peaks are frequently distorted or highly asymmetric. Interaction of charged species with the wall of the capillary is the primary source for serious band broadening and peak tailing. This paper reports a mathematical model for the peak profiles in capillary electrophoresis, taking adsorption on capillary wall into account. The model is based on the advection-diffusion equation, Langmuir second order kinetic equation and appropriate boundary conditions. It is applied to simulate the gel electrophoretic separation of the 11 fragment Φ X174-Hae III double stranded DNA ladders in a polymeric microchip. By using the migration velocities and diffusivities from the measurement, and properly selecting two fitting parameters, namely adsorption and desorption coefficients, the simulated peak shapes show remarkable similarity with the experimental electrophoretic results. The effect of adsorption and desorption coefficients are also investigated and the result shows that adsorption of analytes from the main analyte zone and desorption of these analytes appear to be the reasons of peak tailing, with the latter being the major cause. Springer-Verlag, 2006 Peak tailing nationallicence DNA nationallicence Gel electrophoresis nationallicence Microchip nationallicence Sun Yi National Institute of Education, 1 Nanyang Walk, 637616, Singapore, Singapore aut Kwok Yien National Institute of Education, 1 Nanyang Walk, 637616, Singapore, Singapore aut Nguyen Nam-Trung School of Mechanical and Aerospace Engineering, Nanyang Technological University, 50 Nanyang Avenue, 639798, Singapore, Singapore aut Microfluidics and Nanofluidics Springer-Verlag 3/3(2007-06-01), 323-332 1613-4982 3:3<323 2007 3 10404 https://doi.org/10.1007/s10404-006-0126-3 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10404-006-0126-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Sun Yi National Institute of Education, 1 Nanyang Walk, 637616, Singapore, Singapore aut NATIONALLICENCE 700 1- Kwok Yien National Institute of Education, 1 Nanyang Walk, 637616, Singapore, Singapore aut NATIONALLICENCE 700 1- Nguyen Nam-Trung School of Mechanical and Aerospace Engineering, Nanyang Technological University, 50 Nanyang Avenue, 639798, Singapore, Singapore aut NATIONALLICENCE 773 0- Microfluidics and Nanofluidics Springer-Verlag 3/3(2007-06-01), 323-332 1613-4982 3:3<323 2007 3 10404 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer