caa a22 4500 463235312 CHVBK 20180405153255.0 cr unu---uuuuu 170326e20071001xx s 000 0 eng 10.1007/s10404-007-0153-8 doi (NATIONALLICENCE)springer-10.1007/s10404-007-0153-8 Numerical and experimental study of a droplet-based PCR chip [Elektronische Daten] [S. Mohr, Y.-H. Zhang, A. Macaskill, P. Day, R. Barber, N. Goddard, D. Emerson, P. Fielden] A two-temperature continuous-flow polymerase chain reaction (PCR) polymer chip has been constructed that takes advantage of droplet technology to avoid sample contamination and adsorption at the surface. Samples contained in aqueous droplets are continuously moved by an oil carrier-fluid through various temperature zones, introducing the possibility of real-time quantitative PCR. In the present paper, we investigate many of the factors affecting droplet-based PCR chip design, including thermal mass, flow rate, and thermal resistance. The study focuses particularly on the fluid and substrate temperature distribution within the PCR chip and the droplet residence times in critical temperature zones. The simulations demonstrate that the flow rate strongly affects the temperature field within the carrier-fluid. Above a critical flow rate, the carrier-fluid fails to achieve the required temperatures for DNA amplification. In addition, the thermal resistances of the different layers in the chip are shown to have a major impact on the temperature profile in the channel. Springer-Verlag, 2007 DNA amplification nationallicence PCR chip nationallicence Continuous flow nationallicence Droplet nationallicence Thermal cycling nationallicence Mohr S. School of Chemical Engineering and Analytical Science, University of Manchester, M60 1QD, Manchester, UK aut Zhang Y.-H Centre for Microfluidics and Microsystems Modelling, CCLRC Daresbury Laboratory, WA4 4AD, Warrington, Cheshire, UK aut Macaskill A. School of Chemical Engineering and Analytical Science, University of Manchester, M60 1QD, Manchester, UK aut Day P. Centre for Integrated Genomic Medical Research, University of Manchester, Stopford Building, Oxford Road, M13 9PT, Manchester, UK aut Barber R. Centre for Microfluidics and Microsystems Modelling, CCLRC Daresbury Laboratory, WA4 4AD, Warrington, Cheshire, UK aut Goddard N. School of Chemical Engineering and Analytical Science, University of Manchester, M60 1QD, Manchester, UK aut Emerson D. Centre for Microfluidics and Microsystems Modelling, CCLRC Daresbury Laboratory, WA4 4AD, Warrington, Cheshire, UK aut Fielden P. School of Chemical Engineering and Analytical Science, University of Manchester, M60 1QD, Manchester, UK aut Microfluidics and Nanofluidics Springer-Verlag 3/5(2007-10-01), 611-621 1613-4982 3:5<611 2007 3 10404 https://doi.org/10.1007/s10404-007-0153-8 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10404-007-0153-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Mohr S. School of Chemical Engineering and Analytical Science, University of Manchester, M60 1QD, Manchester, UK aut NATIONALLICENCE 700 1- Zhang Y.-H Centre for Microfluidics and Microsystems Modelling, CCLRC Daresbury Laboratory, WA4 4AD, Warrington, Cheshire, UK aut NATIONALLICENCE 700 1- Macaskill A. School of Chemical Engineering and Analytical Science, University of Manchester, M60 1QD, Manchester, UK aut NATIONALLICENCE 700 1- Day P. Centre for Integrated Genomic Medical Research, University of Manchester, Stopford Building, Oxford Road, M13 9PT, Manchester, UK aut NATIONALLICENCE 700 1- Barber R. Centre for Microfluidics and Microsystems Modelling, CCLRC Daresbury Laboratory, WA4 4AD, Warrington, Cheshire, UK aut NATIONALLICENCE 700 1- Goddard N. School of Chemical Engineering and Analytical Science, University of Manchester, M60 1QD, Manchester, UK aut NATIONALLICENCE 700 1- Emerson D. Centre for Microfluidics and Microsystems Modelling, CCLRC Daresbury Laboratory, WA4 4AD, Warrington, Cheshire, UK aut NATIONALLICENCE 700 1- Fielden P. School of Chemical Engineering and Analytical Science, University of Manchester, M60 1QD, Manchester, UK aut NATIONALLICENCE 773 0- Microfluidics and Nanofluidics Springer-Verlag 3/5(2007-10-01), 611-621 1613-4982 3:5<611 2007 3 10404 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer