caa a22 4500 463235339 CHVBK 20180405153255.0 cr unu---uuuuu 170326e20071001xx s 000 0 eng 10.1007/s10404-006-0142-3 doi (NATIONALLICENCE)springer-10.1007/s10404-006-0142-3 Development of sorting, aligning, and orienting motile sperm using microfluidic device operated by hydrostatic pressure [Elektronische Daten] [Duck-bong Seo, Yuksel Agca, Z. Feng, John Critser] In vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI) are the most commonly used assisted reproductive technologies to overcome male infertility problems. One of the obstacles of IVF and ICSI procedures is separating motile sperm from non-motile sperm to select the most competent sperm population from any given sperm sample. In addition, orientation and separation of the head from the tail is another obstacle for ICSI. Using the self-movement of sperm against flow direction, motile and non-motile sperm can be separated with an inexpensive polymeric microfluidic system. In this paper, we describe the development of a microfluidic system obtained through low-cost fabrication processes. We report experimental results of sperm sorting using hydrostatic pressure of three different species: bull, mouse, and human. The movement of cells in these channels was observed under a microscope and recorded with a digital camera. It is shown that the hydrostatic pressure and self-movement of motile sperm can be used to solve separating, aligning and orienting sperm in the microchannel. Springer-Verlag, 2007 Microfluidics nationallicence Motile-sperm sorting nationallicence Intracytoplasmic sperm injection nationallicence Seo Duck-bong Department of Mechanical and Aerospace Engineering, University of Missouri-Columbia, 65211, Columbia, MO, USA aut Agca Yuksel Department of Veterinary Pathobiology, Comparative Medicine Center, University of Missouri-Columbia, E-109 Veterinary Medicine Bldg, 65211, Columbia, MO, USA aut Feng Z. Department of Mechanical and Aerospace Engineering, University of Missouri-Columbia, 65211, Columbia, MO, USA aut Critser John Department of Veterinary Pathobiology, Comparative Medicine Center, University of Missouri-Columbia, E-109 Veterinary Medicine Bldg, 65211, Columbia, MO, USA aut Microfluidics and Nanofluidics Springer-Verlag 3/5(2007-10-01), 561-570 1613-4982 3:5<561 2007 3 10404 https://doi.org/10.1007/s10404-006-0142-3 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10404-006-0142-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Seo Duck-bong Department of Mechanical and Aerospace Engineering, University of Missouri-Columbia, 65211, Columbia, MO, USA aut NATIONALLICENCE 700 1- Agca Yuksel Department of Veterinary Pathobiology, Comparative Medicine Center, University of Missouri-Columbia, E-109 Veterinary Medicine Bldg, 65211, Columbia, MO, USA aut NATIONALLICENCE 700 1- Feng Z. Department of Mechanical and Aerospace Engineering, University of Missouri-Columbia, 65211, Columbia, MO, USA aut NATIONALLICENCE 700 1- Critser John Department of Veterinary Pathobiology, Comparative Medicine Center, University of Missouri-Columbia, E-109 Veterinary Medicine Bldg, 65211, Columbia, MO, USA aut NATIONALLICENCE 773 0- Microfluidics and Nanofluidics Springer-Verlag 3/5(2007-10-01), 561-570 1613-4982 3:5<561 2007 3 10404 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer