caa a22 4500 463235371 CHVBK 20180405153255.0 cr unu---uuuuu 170326e20071001xx s 000 0 eng 10.1007/s10404-007-0155-6 doi (NATIONALLICENCE)springer-10.1007/s10404-007-0155-6 Automated module for hybridization and staining of commercially produced nucleic acid microarrays [Elektronische Daten] [J. Erickson, J. Hu, G. Anderson, A. Salls, S. Gray, J. Golden, B. Lin, F. Ligler] Nucleic acid microarrays are a rapidly expanding technology that enables the detection of pathogens at the genetic level. Currently, the processing of commercially produced microarrays requires cumbersome, expensive, and time-consuming benchtop equipment, which is not practical for point-of-care diagnostic applications. We demonstrate a portable module that can perform the hybridization, wash, and stain steps required for processing a nucleic acid microarray; and it performs these steps in a timeline significantly shorter than the standard commercial protocol. This device is automated, has a small footprint, and serves as a replacement for two commercial pieces of benchtop equipment. Furthermore, our device is designed to serve as a module in a portable biosensor that performs automated sample preparation and nucleic acid amplification. Results with Affymetrix GeneChips show that our device performs as well as non-portable equipment specifically manufactured to process these microarrays. Springer-Verlag, 2007 Microarray nationallicence Hybridization nationallicence Automated nationallicence Diagnostics nationallicence Portable nationallicence Erickson J. Naval Research Laboratory, Code 6900, Center for Bio/Molecular Science and Engineering, 20375-5438, Washington, DC, USA aut Hu J. Nova Research Inc., 22309, Alexandria, VA, USA aut Anderson G. Naval Research Laboratory, Code 6900, Center for Bio/Molecular Science and Engineering, 20375-5438, Washington, DC, USA aut Salls A. Naval Research Laboratory, Code 6900, Center for Bio/Molecular Science and Engineering, 20375-5438, Washington, DC, USA aut Gray S. Naval Research Laboratory, Code 6900, Center for Bio/Molecular Science and Engineering, 20375-5438, Washington, DC, USA aut Golden J. Naval Research Laboratory, Code 6900, Center for Bio/Molecular Science and Engineering, 20375-5438, Washington, DC, USA aut Lin B. Naval Research Laboratory, Code 6900, Center for Bio/Molecular Science and Engineering, 20375-5438, Washington, DC, USA aut Ligler F. Naval Research Laboratory, Code 6900, Center for Bio/Molecular Science and Engineering, 20375-5438, Washington, DC, USA aut Microfluidics and Nanofluidics Springer-Verlag 3/5(2007-10-01), 623-628 1613-4982 3:5<623 2007 3 10404 https://doi.org/10.1007/s10404-007-0155-6 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10404-007-0155-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Erickson J. Naval Research Laboratory, Code 6900, Center for Bio/Molecular Science and Engineering, 20375-5438, Washington, DC, USA aut NATIONALLICENCE 700 1- Hu J. Nova Research Inc., 22309, Alexandria, VA, USA aut NATIONALLICENCE 700 1- Anderson G. Naval Research Laboratory, Code 6900, Center for Bio/Molecular Science and Engineering, 20375-5438, Washington, DC, USA aut NATIONALLICENCE 700 1- Salls A. Naval Research Laboratory, Code 6900, Center for Bio/Molecular Science and Engineering, 20375-5438, Washington, DC, USA aut NATIONALLICENCE 700 1- Gray S. Naval Research Laboratory, Code 6900, Center for Bio/Molecular Science and Engineering, 20375-5438, Washington, DC, USA aut NATIONALLICENCE 700 1- Golden J. Naval Research Laboratory, Code 6900, Center for Bio/Molecular Science and Engineering, 20375-5438, Washington, DC, USA aut NATIONALLICENCE 700 1- Lin B. Naval Research Laboratory, Code 6900, Center for Bio/Molecular Science and Engineering, 20375-5438, Washington, DC, USA aut NATIONALLICENCE 700 1- Ligler F. Naval Research Laboratory, Code 6900, Center for Bio/Molecular Science and Engineering, 20375-5438, Washington, DC, USA aut NATIONALLICENCE 773 0- Microfluidics and Nanofluidics Springer-Verlag 3/5(2007-10-01), 623-628 1613-4982 3:5<623 2007 3 10404 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer