caa a22 4500 46323541X CHVBK 20180405153255.0 cr unu---uuuuu 170326e20071001xx s 000 0 eng 10.1007/s10404-006-0140-5 doi (NATIONALLICENCE)springer-10.1007/s10404-006-0140-5 Non-contact printing of proteins on reactive polymer surfaces: a novel route towards structured and graded cell culture carriers [Elektronische Daten] [M. Herklotz, M. Melamed, C. Trautmann, M. Nitschke, T. Pompe, F. Gast, F. Howitz, C. Werner] Non-contact printing with a GeSiM Nano-PlotterTM was applied to prepare cell culture carriers with lateral structures of different proteins bound to a reactive surface of poly(ethylene-alt-maleic anhydride). As a proof of principle, it was shown that protein microstructures obtained by this technique can direct the behavior of human endothelial cells. The cells adhere and proliferate on collagen type IV spots provided by the microstructured substrate. Since non-contact printing allows us to prepare a multitude of protein spots with different compositions and concentrations on one and the same reactive cell culture carrier, multi-parameter studies on the role of proteins and its anchorage to the substrate can be carried out in an effective way. Springer-Verlag, 2007 Non-contact printing nationallicence Micropatterning nationallicence Cell adhesion nationallicence Herklotz M. Leibniz Institute of Polymer Research Dresden, Max Bergmann Center of Biomaterials Dresden, Hohe Str. 6, 01069, Dresden, Germany aut Melamed M. Leibniz Institute of Polymer Research Dresden, Max Bergmann Center of Biomaterials Dresden, Hohe Str. 6, 01069, Dresden, Germany aut Trautmann C. Leibniz Institute of Polymer Research Dresden, Max Bergmann Center of Biomaterials Dresden, Hohe Str. 6, 01069, Dresden, Germany aut Nitschke M. Leibniz Institute of Polymer Research Dresden, Max Bergmann Center of Biomaterials Dresden, Hohe Str. 6, 01069, Dresden, Germany aut Pompe T. Leibniz Institute of Polymer Research Dresden, Max Bergmann Center of Biomaterials Dresden, Hohe Str. 6, 01069, Dresden, Germany aut Gast F. GeSiM mbH, Bautzner Landstraße 45, 01454, Großerkmannsdorf, Germany aut Howitz F. GeSiM mbH, Bautzner Landstraße 45, 01454, Großerkmannsdorf, Germany aut Werner C. Leibniz Institute of Polymer Research Dresden, Max Bergmann Center of Biomaterials Dresden, Hohe Str. 6, 01069, Dresden, Germany aut Microfluidics and Nanofluidics Springer-Verlag 3/5(2007-10-01), 629-633 1613-4982 3:5<629 2007 3 10404 https://doi.org/10.1007/s10404-006-0140-5 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10404-006-0140-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Herklotz M. Leibniz Institute of Polymer Research Dresden, Max Bergmann Center of Biomaterials Dresden, Hohe Str. 6, 01069, Dresden, Germany aut NATIONALLICENCE 700 1- Melamed M. Leibniz Institute of Polymer Research Dresden, Max Bergmann Center of Biomaterials Dresden, Hohe Str. 6, 01069, Dresden, Germany aut NATIONALLICENCE 700 1- Trautmann C. Leibniz Institute of Polymer Research Dresden, Max Bergmann Center of Biomaterials Dresden, Hohe Str. 6, 01069, Dresden, Germany aut NATIONALLICENCE 700 1- Nitschke M. Leibniz Institute of Polymer Research Dresden, Max Bergmann Center of Biomaterials Dresden, Hohe Str. 6, 01069, Dresden, Germany aut NATIONALLICENCE 700 1- Pompe T. Leibniz Institute of Polymer Research Dresden, Max Bergmann Center of Biomaterials Dresden, Hohe Str. 6, 01069, Dresden, Germany aut NATIONALLICENCE 700 1- Gast F. GeSiM mbH, Bautzner Landstraße 45, 01454, Großerkmannsdorf, Germany aut NATIONALLICENCE 700 1- Howitz F. GeSiM mbH, Bautzner Landstraße 45, 01454, Großerkmannsdorf, Germany aut NATIONALLICENCE 700 1- Werner C. Leibniz Institute of Polymer Research Dresden, Max Bergmann Center of Biomaterials Dresden, Hohe Str. 6, 01069, Dresden, Germany aut NATIONALLICENCE 773 0- Microfluidics and Nanofluidics Springer-Verlag 3/5(2007-10-01), 629-633 1613-4982 3:5<629 2007 3 10404 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer