caa a22 4500 463235533 CHVBK 20180405153255.0 cr unu---uuuuu 170326e20070401xx s 000 0 eng 10.1007/s10404-006-0114-7 doi (NATIONALLICENCE)springer-10.1007/s10404-006-0114-7 An integrated genetic analysis microfluidic platform with valves and a PCR chip reusability method to avoid contamination [Elektronische Daten] [Ranjit Prakash, Karan Kaler] Clinical diagnostics and genomic research often require performing numerous genetic tests. While microfluidic devices provide a low-cost alternative to such demands, integrated microfluidic devices are fabricated using expensive technology not always affordable for single use. However, carryover cross-contamination (CXC) concerns (i.e. either false positive or false negative PCR data) in PCR chips prevent reuse, defying much of the advantages of miniaturized systems developed using expensive MEMS processing. In this work, we present an integrated and reusable PCR-CE glass microfluidic chip capable of multi-chamber PCR and sequential CE, with emphasis on a unique chip reusability approach to avoid CXC. For reliable PCR, the surface of the chamber is re-configured from its virgin hydrophilic (CA<20°) to hydrophobic (CA>110°) by silanization. To then extend this silanization method as a chip reusability technique, the silanization coating is ‘stripped and re-silanized' (SRS) to create a fresh coating prior to each successive PCR run. Experimental confirmation of the effectiveness of SRS method in avoiding the CXC is demonstrated using plasmid DNA and HIV-1 infected DNA samples. We also present passive plug microvalves incorporated in the chip to enable fluid/vapor retention during the PCR and controlled fluid flow from the PCR chamber to the CE section for further analysis. Springer-Verlag, 2006 PCR nationallicence CE nationallicence Integrated nationallicence Reuse nationallicence Valves nationallicence Prakash Ranjit Biosystems Research and Applications Group, Schulich School of Engineering, University of Calgary, 2500 University Drive NW, T2N 1N4, Calgary, AB, Canada aut Kaler Karan Biosystems Research and Applications Group, Schulich School of Engineering, University of Calgary, 2500 University Drive NW, T2N 1N4, Calgary, AB, Canada aut Microfluidics and Nanofluidics Springer-Verlag 3/2(2007-04-01), 177-187 1613-4982 3:2<177 2007 3 10404 https://doi.org/10.1007/s10404-006-0114-7 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10404-006-0114-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Prakash Ranjit Biosystems Research and Applications Group, Schulich School of Engineering, University of Calgary, 2500 University Drive NW, T2N 1N4, Calgary, AB, Canada aut NATIONALLICENCE 700 1- Kaler Karan Biosystems Research and Applications Group, Schulich School of Engineering, University of Calgary, 2500 University Drive NW, T2N 1N4, Calgary, AB, Canada aut NATIONALLICENCE 773 0- Microfluidics and Nanofluidics Springer-Verlag 3/2(2007-04-01), 177-187 1613-4982 3:2<177 2007 3 10404 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer