caa a22 4500 46323555X CHVBK 20180405153255.0 cr unu---uuuuu 170326e20070101xx s 000 0 eng 10.1007/s11240-006-9183-2 doi (NATIONALLICENCE)springer-10.1007/s11240-006-9183-2 Mutation induced by ethylmethanesulphonate (EMS), in vitro screening for salt tolerance and plant regeneration of sweet potato ( Ipomoea batatas L.) [Elektronische Daten] [Yu-Shi Luan, Juan Zhang, Xiao-Rong Gao, Li-Jia An] Salt tolerant cultivars of sweet potato (Ipomoea batatas L.) can be obtained from induced mutation. The objective of the present study was to induce mutation for salt tolerance using ethylmethanesulphonate (EMS) in calli of sweet potato, followed by cell line selection and subsequent plant regeneration. Calli initiated from leaf explants were treated with 0.5% EMS for 0, 1, 1.5, 2, 2.5 and 3h, followed by rinsing with sterile distilled water for four times. Preliminary experiments showed that 200mM NaCl could be used as selection pressure. Salt tolerant calli were sub-cultured on medium supplemented with 200mM NaCl for selection of mutant cell lines and this process repeated 5times (20days each). The selected calli were transferred onto somatic embryo formation medium, which was Murashige and Skoog (MS) medium supplemented with 4mgl−1 abscisic acid (ABA), 10mgl−1 gibberellic acid (GA). After 15days, somatic embryos were transferred onto MS medium supplemented with 0.05mgl−1 ABA, 0.2mgl−1 zeatin (ZT) for regeneration. Plants designated as ML1, ML2and ML3 were regenerated from the somatic embryos formed by calli treated with 0.5% EMS for 2 and 2.5h. After propagation, salt tolerance of these mutants was investigated. Data suggested the mutants were more salt tolerant than control plants. Springer Science+Business Media B.V., 2006 Sweet potato nationallicence Calli nationallicence Mutagenesis nationallicence Mutant nationallicence Salt tolerance nationallicence EMS : Ethylmethanesulphonate nationallicence MS : Murashige and Skoog nationallicence ABA : Abscisic acid nationallicence GA : Gibberellic acid nationallicence ZT : Zeatin nationallicence 4FA : 4-fluorophenoxyacetic acid nationallicence Luan Yu-Shi Department of Bioscience and Bioengineering, Dalian University of Technology, 116024, Dalian, China aut Zhang Juan Department of Bioscience and Bioengineering, Dalian University of Technology, 116024, Dalian, China aut Gao Xiao-Rong Department of Bioscience and Bioengineering, Dalian University of Technology, 116024, Dalian, China aut An Li-Jia Department of Bioscience and Bioengineering, Dalian University of Technology, 116024, Dalian, China aut Plant Cell, Tissue and Organ Culture Springer Netherlands 88/1(2007-01-01), 77-81 0167-6857 88:1<77 2007 88 11240 https://doi.org/10.1007/s11240-006-9183-2 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11240-006-9183-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Luan Yu-Shi Department of Bioscience and Bioengineering, Dalian University of Technology, 116024, Dalian, China aut NATIONALLICENCE 700 1- Zhang Juan Department of Bioscience and Bioengineering, Dalian University of Technology, 116024, Dalian, China aut NATIONALLICENCE 700 1- Gao Xiao-Rong Department of Bioscience and Bioengineering, Dalian University of Technology, 116024, Dalian, China aut NATIONALLICENCE 700 1- An Li-Jia Department of Bioscience and Bioengineering, Dalian University of Technology, 116024, Dalian, China aut NATIONALLICENCE 773 0- Plant Cell, Tissue and Organ Culture Springer Netherlands 88/1(2007-01-01), 77-81 0167-6857 88:1<77 2007 88 11240 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer