caa a22 4500 463235568 CHVBK 20180405153255.0 cr unu---uuuuu 170326e20070101xx s 000 0 eng 10.1007/s11240-006-9178-z doi (NATIONALLICENCE)springer-10.1007/s11240-006-9178-z In-vitro development of ovules obtained after pollination of cotton ( Gossypium spp) flowers with pollen from okra ( Abelmoschus esculentus L. Moench) [Elektronische Daten] [D. Vlachostergios, A. Mavromatis, S. Kantartzi, D. Roupakias] The in vitro response of ovules obtained after pollination of cotton flowers with pollen from Abelmoschus esculentus was studied. For this, 492 cotton flowers from five G. hirsutum varieties, four G. barbadense varieties and 10 F1 interspecific hybrids, were pollinated with pollen from A. esculentus and 5,069 ovules were cultured in vitro. From the cultured ovules, 69 embryos were isolated and 16 of them grew into plants. However, only three of them survived after transplantation. Finally, one plant which originated from the interspecific cross (B403×Acala Sindos)×A. esculentus reached maturity. The mature plant (Pa0) had no morphological traits from A. esculentus. On the contrary, traits from both cotton species were observed. The flowcytometric analysis of the Pa0 plant indicated that it was hypoaneuploid. Root tip chromosome counts of its offsprings revealed a progressive chromosome increase from the Pa1 to Pa4 generation. Plants with 52 chromosomes or hypoaneuploids with a lower level of chromosomes (46-51) could be isolated from the Pa4 generation. These plants exhibited morphological traits from both cotton species and they were fertile. No signs of A. esculentus morphological characteristics were observed in these plants. It was concluded that aneuploid partial interspecific cotton plants could be produced after pollination of cotton interspecific hybrids with pollen from A. esculentus and application of an in-ovule embryo rescue technique. Springer Science+Business Media B.V., 2006 Abelmoschus esculentus nationallicence Aneuploid partial interspecific cotton plants nationallicence Intergeneric pollination nationallicence Genetic analysis nationallicence IAA : Indole-3-acetic acid nationallicence KIN : 6-Furfurylaminopurine nationallicence GA3 : Gibberellic acid A3 nationallicence NAA : 1-Naphthaleneacetic acid nationallicence DAP : Days after pollination nationallicence DAC : Days in culture nationallicence MS : Murashige and Skoog medium (1962) nationallicence SH : Stewart and Hsu medium (1977) nationallicence Vlachostergios D. Department of Genetics and Plant Breeding, School of Agriculture, Aristotle University of Thessaloniki, 54124, Thessaloniki, Greece aut Mavromatis A. Department of Crop Production & Rural Environment, School of Agricultural Sciences, University of Thessaly, 38446, Volos, Greece aut Kantartzi S. Department of Genetics and Plant Breeding, School of Agriculture, Aristotle University of Thessaloniki, 54124, Thessaloniki, Greece aut Roupakias D. Department of Genetics and Plant Breeding, School of Agriculture, Aristotle University of Thessaloniki, 54124, Thessaloniki, Greece aut Plant Cell, Tissue and Organ Culture Springer Netherlands 88/1(2007-01-01), 109-115 0167-6857 88:1<109 2007 88 11240 https://doi.org/10.1007/s11240-006-9178-z text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11240-006-9178-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Vlachostergios D. Department of Genetics and Plant Breeding, School of Agriculture, Aristotle University of Thessaloniki, 54124, Thessaloniki, Greece aut NATIONALLICENCE 700 1- Mavromatis A. Department of Crop Production & Rural Environment, School of Agricultural Sciences, University of Thessaly, 38446, Volos, Greece aut NATIONALLICENCE 700 1- Kantartzi S. Department of Genetics and Plant Breeding, School of Agriculture, Aristotle University of Thessaloniki, 54124, Thessaloniki, Greece aut NATIONALLICENCE 700 1- Roupakias D. Department of Genetics and Plant Breeding, School of Agriculture, Aristotle University of Thessaloniki, 54124, Thessaloniki, Greece aut NATIONALLICENCE 773 0- Plant Cell, Tissue and Organ Culture Springer Netherlands 88/1(2007-01-01), 109-115 0167-6857 88:1<109 2007 88 11240 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer