caa a22 4500 463235576 CHVBK 20180405153255.0 cr unu---uuuuu 170326e20070101xx s 000 0 eng 10.1007/s11240-006-9116-0 doi (NATIONALLICENCE)springer-10.1007/s11240-006-9116-0 Adventitious plant regeneration on leaf explants from adult male kiwifruit and AFLP analysis of genetic variation [Elektronische Daten] [M. Prado, M. Gonzalez, S. Romo, M. Herrera] The present work reports on a study of plant regeneration carried out with callus from the leaf blades and petioles of field-grown male adult kiwifruit plants (Actinidia deliciosa (Chev.) Liang and Ferguson). The cultivars used were ‘Tomuri' and clone A, a selected male plant grown in north western Spain. The best shoot induction conditions were obtained in ‘Tomuri' leaf blades cultured in K(h) medium in the presence of 23µM Zeatin and 0.1µM NAA. Under these conditions, more than 80% of organogenic callus induction was observed, with an average of 14 new shoots in the second subculture. The initial length of the shoots affected shoot elongation, which was accomplished by culturing isolated shoots in K(h) medium with half-strength salts, supplemented with 0.4µM Zeatin and 0.1µM NAA. A possible detrimental long-term effect of cytokinins on shoot elongation can account for the results, since elongation was not observed until 1month of culture in elongation medium. For rooting, shoots (1cm in length) were basally immersed in a 5mM IBA solution for 15s, and transferred to half-strength K(h) basal medium. Regenerated plants were acclimated in a sterile peat:perlite substrate for 10days, and then transferred to soil. AFLP analysis was accomplished with 15 primer combinations from which 13 showed reproducible and well-resolved bands, producing a total of 1321 fragments from which 1281 were polymorphic (97%). A dendrogram was constructed using both monomorphic and polymorphic bands, showing genetic variation among field-grown plants and tissue culture-derived regenerants. Springer Science+Business Media B.V., 2006 ‘Tomuri' nationallicence Actinidia deliciosa nationallicence Tissue culture nationallicence Somaclonal variation nationallicence Molecular markers nationallicence AFLP : Amplified Restriction Fragment Length Polymorphism nationallicence BA : N6-benzyladenine nationallicence CTAB : Hexadecyltrimethylammonium bromide nationallicence IBA : Indole-3-butyric acid nationallicence K(h) : Cheng (1975) salts and vitamins nationallicence KIN : Kinetin nationallicence NAA : 1-Naphthaleneacetic acid nationallicence RAPD : Random Amplified Polymorphic DNA nationallicence SSR : Simple Sequence Repeat nationallicence UPGMA : Unweighted Pair-Group Method Arithmetic averages nationallicence Z : Zeatin nationallicence Prado M. Dpto. Fisiología Vegetal, Escuela Politécnica Superior, Campus de Lugo, 27002, Lugo, Spain aut Gonzalez M. Dpto. Fisiología Vegetal, Escuela Politécnica Superior, Campus de Lugo, 27002, Lugo, Spain aut Romo S. Dpto. Fisiología Vegetal, Escuela Politécnica Superior, Campus de Lugo, 27002, Lugo, Spain aut Herrera M. Dpto. Fisiología Vegetal, Escuela Politécnica Superior, Campus de Lugo, 27002, Lugo, Spain aut Plant Cell, Tissue and Organ Culture Springer Netherlands 88/1(2007-01-01), 1-10 0167-6857 88:1<1 2007 88 11240 https://doi.org/10.1007/s11240-006-9116-0 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11240-006-9116-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Prado M. Dpto. Fisiología Vegetal, Escuela Politécnica Superior, Campus de Lugo, 27002, Lugo, Spain aut NATIONALLICENCE 700 1- Gonzalez M. Dpto. Fisiología Vegetal, Escuela Politécnica Superior, Campus de Lugo, 27002, Lugo, Spain aut NATIONALLICENCE 700 1- Romo S. Dpto. Fisiología Vegetal, Escuela Politécnica Superior, Campus de Lugo, 27002, Lugo, Spain aut NATIONALLICENCE 700 1- Herrera M. Dpto. Fisiología Vegetal, Escuela Politécnica Superior, Campus de Lugo, 27002, Lugo, Spain aut NATIONALLICENCE 773 0- Plant Cell, Tissue and Organ Culture Springer Netherlands 88/1(2007-01-01), 1-10 0167-6857 88:1<1 2007 88 11240 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer