caa a22 4500 463235584 CHVBK 20180405153255.0 cr unu---uuuuu 170326e20070101xx s 000 0 eng 10.1007/s11240-006-9162-7 doi (NATIONALLICENCE)springer-10.1007/s11240-006-9162-7 Transgene expression in Chinese sweetgum driven by the salt induced expressed promoter [Elektronische Daten] [Zhuo Renying, Qiao Guirong, Sun Zongxiu] Conventional breeding of Chinese sweetgum is constrained by its long-reproductive cycle, which includes long-juvenile periods, and by its complex reproductive characteristics, including self-incompatibility and a high degree of heterozygosis. Like other tree species, sweetgum has undergone relatively little domestication; the methodology described here in illustrates the possibility of transforming Liquidambar formosana L. obtained from leafy explants using Agrobacter tumefaciens. PCR and Southern blotting show that foreign gene had integrated to genomic DNA. The results indicated that superoxide dismutase (SOD) and peroxidase (POD) activities increased with the stress time in all treated plants and these activities of the transgenic plant were stably higher than those of the control. RT-PCR showed that BADH expressed strongly induced by NaCl. The present study showed that the Rd29A promoter is able to direct osmotant gene expression when plant was exposed to salt, cold, and drought stress, with the advantage that expression was absent or undetectable in natural grow phase. Springer Science+Business Media B.V., 2006 Chinese sweetgum nationallicence Plantlet regeneration nationallicence Transgenic plantlet nationallicence Salt induced promoter nationallicence Renying Zhuo Key lab of tree genomics, The Research Institute of Subtropical of Forest, Chinese Academy of Forest, 311400, Fuyang, Hangzhou Zhejiang, China aut Guirong Qiao Key lab of tree genomics, The Research Institute of Subtropical of Forest, Chinese Academy of Forest, 311400, Fuyang, Hangzhou Zhejiang, China aut Zongxiu Sun State Key Laboratory of Rice Biology, China National Rice Research Institute, 310006, Hangzhou, Zhejiang, China aut Plant Cell, Tissue and Organ Culture Springer Netherlands 88/1(2007-01-01), 101-107 0167-6857 88:1<101 2007 88 11240 https://doi.org/10.1007/s11240-006-9162-7 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11240-006-9162-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Renying Zhuo Key lab of tree genomics, The Research Institute of Subtropical of Forest, Chinese Academy of Forest, 311400, Fuyang, Hangzhou Zhejiang, China aut NATIONALLICENCE 700 1- Guirong Qiao Key lab of tree genomics, The Research Institute of Subtropical of Forest, Chinese Academy of Forest, 311400, Fuyang, Hangzhou Zhejiang, China aut NATIONALLICENCE 700 1- Zongxiu Sun State Key Laboratory of Rice Biology, China National Rice Research Institute, 310006, Hangzhou, Zhejiang, China aut NATIONALLICENCE 773 0- Plant Cell, Tissue and Organ Culture Springer Netherlands 88/1(2007-01-01), 101-107 0167-6857 88:1<101 2007 88 11240 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer