caa a22 4500 463235592 CHVBK 20180405153255.0 cr unu---uuuuu 170326e20070101xx s 000 0 eng 10.1007/s11240-006-9179-y doi (NATIONALLICENCE)springer-10.1007/s11240-006-9179-y In vitro morphogenesis from cotyledon and epicotyl explants and flow cytometry distinction between landraces of Bambara groundnut [ Vigna subterranea (L.) Verdc], an under-utilised grain legume [Elektronische Daten] [M. Koné, E. Patat-Ochatt, C. Conreux, R. Sangwan, S. Ochatt] The morphogenetic competence of Bambara groundnut was assessed for different landraces, explant sources and media compositions. With cotyledon explants, the best callusing occurred on a medium containing 3mg/l BAP + 0.5mg/l NAA, while roots were produced with 3-5mg/l BAP+0.5mg/l NAA. Shoots regenerated (∼6%) from cotyledons on media with BAP alone (3-5mg/l) or combined with 0.01-0.1mg/l NAA. Flowers were regenerated on 5mg/l BAP+0.5mg/l NAA, without any intervening callus phase. With epicotyls, the highest callusing was on 3mg/l BAP+0.5mg/l NAA, and shoots regenerated (15-20%) on 3mg/l BAP alone or with NAA at concentrations that depended on the landrace studied. Regenerated shoots rooted on hormone-free medium, and plants transferred to the greenhouse were all morphologically normal and fertile. Flow cytometry showed that most regenerants were diploid and in addition permitted to distinguish between landraces according to their relative nuclear DNA content. This is the first report on de novo regeneration in vitro of Bambara groundnut, an important yet neglected legume crop. Springer Science+Business Media B.V., 2006 Bambara groundnut nationallicence Grain legumes nationallicence Regeneration nationallicence Flow cytometry nationallicence Neglected crops nationallicence Koné M. INRA, C. R. de Dijon, URLEG, PCMV, B.P. 86510, 21065, Dijon Cedex, France aut Patat-Ochatt E. INRA, C. R. de Dijon, URLEG, PCMV, B.P. 86510, 21065, Dijon Cedex, France aut Conreux C. INRA, C. R. de Dijon, URLEG, PCMV, B.P. 86510, 21065, Dijon Cedex, France aut Sangwan R. EA3900, Laboratoire Androgenèse et Biotechnologie, Université de Picardie Jules Verne, 33 rue Saint Leu, 80039, Amiens, France aut Ochatt S. INRA, C. R. de Dijon, URLEG, PCMV, B.P. 86510, 21065, Dijon Cedex, France aut Plant Cell, Tissue and Organ Culture Springer Netherlands 88/1(2007-01-01), 61-75 0167-6857 88:1<61 2007 88 11240 https://doi.org/10.1007/s11240-006-9179-y text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11240-006-9179-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Koné M. INRA, C. R. de Dijon, URLEG, PCMV, B.P. 86510, 21065, Dijon Cedex, France aut NATIONALLICENCE 700 1- Patat-Ochatt E. INRA, C. R. de Dijon, URLEG, PCMV, B.P. 86510, 21065, Dijon Cedex, France aut NATIONALLICENCE 700 1- Conreux C. INRA, C. R. de Dijon, URLEG, PCMV, B.P. 86510, 21065, Dijon Cedex, France aut NATIONALLICENCE 700 1- Sangwan R. EA3900, Laboratoire Androgenèse et Biotechnologie, Université de Picardie Jules Verne, 33 rue Saint Leu, 80039, Amiens, France aut NATIONALLICENCE 700 1- Ochatt S. INRA, C. R. de Dijon, URLEG, PCMV, B.P. 86510, 21065, Dijon Cedex, France aut NATIONALLICENCE 773 0- Plant Cell, Tissue and Organ Culture Springer Netherlands 88/1(2007-01-01), 61-75 0167-6857 88:1<61 2007 88 11240 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer