caa a22 4500 463235606 CHVBK 20180405153255.0 cr unu---uuuuu 170326e20070101xx s 000 0 eng 10.1007/s11240-006-9177-0 doi (NATIONALLICENCE)springer-10.1007/s11240-006-9177-0 Control of shoot necrosis and plant death during micro-propagation of banana and plantains ( Musa spp.) [Elektronische Daten] [Kottackal Martin, Chun-Lai Zhang, Adrian Slater, Joseph Madassery] Shoot necrosis and plant death occurred during large-scale propagation of Musa cultivars, viz. Grande Naine (AAA), Dwarf Cavendish (AAA), Nendran (AAB) and Quintal Nendran (AAB). The timing and frequency of necrosis was influenced by the cultivar. Grande Naine and Dwarf Cavendish showed necrosis after the 7th subculture at frequencies of 27 and 29%, whilst at the rooting stage (after seven transfers of shoot multiplication) the frequencies were 18 and 19% respectively. In Nendran and Quintal Nendran, necrosis occurred after the 5th subculture at frequencies of 38 and 40%, and at the rooting stage (after five transfers of shoot multiplication) the frequencies of necrosis were 26 and 27% respectively. Several methods were tested for alleviating shoot necrosis, including shortening the culture period, altering the media salt strength, use of various plant growth regulators, different levels of sucrose, fructose, silver nitrate, and increasing the concentration of calcium chloride. Only the addition of calcium chloride proved effective in reducing shoot necrosis. Transfer of shoots showing early signs of necrosis to Murashige and Skoog medium with an addition of 50-100mg l-1 calcium chloride facilitated recovery of more than 90% of the shoots. The same addition to shoot multiplication medium for 2-4 transfers after the 6th and 4th subcultures respectively, for bananas and plantains alleviated shoot necrosis up to the 12th subculture in all cultivars. This also prevented necrosis during subsequent rooting. The prevention of loss of cultures by shoot necrosis by the addition of calcium chloride at the shoot multiplication stage, and thereby the reduction of production cost, will be beneficial to the banana micro-propagation industry. Springer Science+Business Media B.V., 2006 Bananas nationallicence Calcium chloride nationallicence Leaf burn nationallicence Plantains nationallicence Shoot necrosis nationallicence BA-N6 : benzyladenine nationallicence IBA : Indole-3-butyric acid nationallicence MS : Murashige and Skoog nationallicence NAA : α-naphthaleneacetic acid nationallicence PGRs : Plant growth regulators nationallicence Martin Kottackal Department of Biotechnology, University of Calicut, 673 635, Calicut, Kerala, India aut Zhang Chun-Lai Norman Borlaug Institute for Plant Science Research, Faculty of Health and Life Sciences, De Montfort University, Hawthorn Building, The Gate Way, LE1 9BH, Leicester, UK aut Slater Adrian Norman Borlaug Institute for Plant Science Research, Faculty of Health and Life Sciences, De Montfort University, Hawthorn Building, The Gate Way, LE1 9BH, Leicester, UK aut Madassery Joseph Department of Biotechnology, University of Calicut, 673 635, Calicut, Kerala, India aut Plant Cell, Tissue and Organ Culture Springer Netherlands 88/1(2007-01-01), 51-59 0167-6857 88:1<51 2007 88 11240 https://doi.org/10.1007/s11240-006-9177-0 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11240-006-9177-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Martin Kottackal Department of Biotechnology, University of Calicut, 673 635, Calicut, Kerala, India aut NATIONALLICENCE 700 1- Zhang Chun-Lai Norman Borlaug Institute for Plant Science Research, Faculty of Health and Life Sciences, De Montfort University, Hawthorn Building, The Gate Way, LE1 9BH, Leicester, UK aut NATIONALLICENCE 700 1- Slater Adrian Norman Borlaug Institute for Plant Science Research, Faculty of Health and Life Sciences, De Montfort University, Hawthorn Building, The Gate Way, LE1 9BH, Leicester, UK aut NATIONALLICENCE 700 1- Madassery Joseph Department of Biotechnology, University of Calicut, 673 635, Calicut, Kerala, India aut NATIONALLICENCE 773 0- Plant Cell, Tissue and Organ Culture Springer Netherlands 88/1(2007-01-01), 51-59 0167-6857 88:1<51 2007 88 11240 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer