caa a22 4500 463235614 CHVBK 20180405153255.0 cr unu---uuuuu 170326e20070101xx s 000 0 eng 10.1007/s11240-006-9176-1 doi (NATIONALLICENCE)springer-10.1007/s11240-006-9176-1 Encapsulation of moss buds: an efficient method for the in vitro conservation and regeneration of the endangered moss Splachnum ampullaceum [Elektronische Daten] [Rubén Mallón, Paula Barros, Asteria Luzardo, María González] In vitro culture techniques are usually employed for ex situ conservation of endangered plant species. However, encapsulation to preserve threatened bryophytes is scarcely used, and only as a pretreatment prior to cryopreservation. In our study, two different methods of germplasm conservation, involving calcium-alginate encapsulation of moss material, were assessed. The plant material used was gametophyte buds (gametophores) of Splachnum ampullaceum Hedw., a rare species of moss. Moss regeneration was evaluated at different periods of time to examine the efficacy of the technique for moss germplasm conservation. The effects of encapsulation and cold storage on developmental parameters such as protonematal colony diameter, bud length, and number of buds were also studied. The results suggest that moss encapsulation with no prior treatment may be a suitable method for germplasm conservation during long periods of time. With our method survival rates as high as 50% could be reached after 2.5years of cold storage versus 0% of 24-h cryopreserved beads. This technique together with cold storage, avoiding freezing, may be especially important in desiccation intolerant mosses. Springer Science+Business Media B.V., 2006 Bryophyte nationallicence Calcium-alginate beads nationallicence Germplasm conservation nationallicence Mallón Rubén Departamento de Fisiología Vegetal, Universidad de Santiago de Compostela, Santiago de Compostela, Spain aut Barros Paula Departamento de Fisiología Vegetal, Universidad de Santiago de Compostela, Santiago de Compostela, Spain aut Luzardo Asteria Departamento de Farmacia y Tecnología Farmacéutica, Universidad de Santiago de Compostela, Santiago de Compostela, Spain aut González María Departamento de Fisiología Vegetal, Universidad de Santiago de Compostela, Santiago de Compostela, Spain aut Plant Cell, Tissue and Organ Culture Springer Netherlands 88/1(2007-01-01), 41-49 0167-6857 88:1<41 2007 88 11240 https://doi.org/10.1007/s11240-006-9176-1 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11240-006-9176-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Mallón Rubén Departamento de Fisiología Vegetal, Universidad de Santiago de Compostela, Santiago de Compostela, Spain aut NATIONALLICENCE 700 1- Barros Paula Departamento de Fisiología Vegetal, Universidad de Santiago de Compostela, Santiago de Compostela, Spain aut NATIONALLICENCE 700 1- Luzardo Asteria Departamento de Farmacia y Tecnología Farmacéutica, Universidad de Santiago de Compostela, Santiago de Compostela, Spain aut NATIONALLICENCE 700 1- González María Departamento de Fisiología Vegetal, Universidad de Santiago de Compostela, Santiago de Compostela, Spain aut NATIONALLICENCE 773 0- Plant Cell, Tissue and Organ Culture Springer Netherlands 88/1(2007-01-01), 41-49 0167-6857 88:1<41 2007 88 11240 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer