caa a22 4500 463235622 CHVBK 20180405153255.0 cr unu---uuuuu 170326e20070101xx s 000 0 eng 10.1007/s11240-006-9165-4 doi (NATIONALLICENCE)springer-10.1007/s11240-006-9165-4 Isolation and characterization of Brittle2 promoter from Zea Mays and its comparison with Ze19 promoter in transgenic tobacco plants [Elektronische Daten] [Xiaoping Chen, Zhangying Wang, Jianhua Wang, Maoyan Wang, Li Zhao, Guoying Wang] ADP-glucose pyrophosphorylase (AGPase) represents a key regulatory step in starch synthesis. A 0.9kb of 5′ flanking region preceding Brittle2 gene, encoding the small subunit of maize endosperm AGPase, was cloned from maize genome and its expression pattern was studied via the expression of β-glucuronidase (GUS) gene in transgenic tobacco. Analysis of GUS activities showed that the 0.9kb fragment flanking Brittle2 gene was sufficient for driving the seed-preferred expression of the reporter gene. The activity of the 0.9kb 5′ flanking fragment was compared with that of the tandem promoter region from a zein gene (zE19, encoding a maize 19kDa zein protein). The results indicated that both promoters were seed-preferred in a dicotyledonous system as tobacco and the activity of zE19 promoter was three to fourfold higher than that of the 0.9kb fragment flanking Brittle2 gene in transgenic tobacco seeds. At the same time, zE19-driven GUS gene expressed earlier than Brittle2 promoter during seed development. Histochemical location of GUS activity indicated that both promoters showed high expression in embryos, which is different from similar promoters tested in maize. Springer Science+Business Media B.V., 2006 Promoter nationallicence Seed-preferred expression nationallicence Maize nationallicence Transgenic tobacco nationallicence CaMV : Cauliflower mosaic virus nationallicence DAF : Days after flowering nationallicence 4-MU : 4-methylumbelliferone nationallicence GUS : β-glucuronidase nationallicence MUG : 4-methylumbelliferyl-β-d-glucuronide nationallicence X-gluc : 5-bromo-4-chloro-3-indolyl-β-d-glucuronide nationallicence Chen Xiaoping State Key Laboratory of Agrobiotechnology and National Center for Maize Improvement, China Agricultural University, 100094, Beijing, China aut Wang Zhangying State Key Laboratory of Agrobiotechnology and National Center for Maize Improvement, China Agricultural University, 100094, Beijing, China aut Wang Jianhua State Key Laboratory of Agrobiotechnology and National Center for Maize Improvement, China Agricultural University, 100094, Beijing, China aut Wang Maoyan State Key Laboratory of Agrobiotechnology and National Center for Maize Improvement, China Agricultural University, 100094, Beijing, China aut Zhao Li State Key Laboratory of Agrobiotechnology and National Center for Maize Improvement, China Agricultural University, 100094, Beijing, China aut Wang Guoying State Key Laboratory of Agrobiotechnology and National Center for Maize Improvement, China Agricultural University, 100094, Beijing, China aut Plant Cell, Tissue and Organ Culture Springer Netherlands 88/1(2007-01-01), 11-20 0167-6857 88:1<11 2007 88 11240 https://doi.org/10.1007/s11240-006-9165-4 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11240-006-9165-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Chen Xiaoping State Key Laboratory of Agrobiotechnology and National Center for Maize Improvement, China Agricultural University, 100094, Beijing, China aut NATIONALLICENCE 700 1- Wang Zhangying State Key Laboratory of Agrobiotechnology and National Center for Maize Improvement, China Agricultural University, 100094, Beijing, China aut NATIONALLICENCE 700 1- Wang Jianhua State Key Laboratory of Agrobiotechnology and National Center for Maize Improvement, China Agricultural University, 100094, Beijing, China aut NATIONALLICENCE 700 1- Wang Maoyan State Key Laboratory of Agrobiotechnology and National Center for Maize Improvement, China Agricultural University, 100094, Beijing, China aut NATIONALLICENCE 700 1- Zhao Li State Key Laboratory of Agrobiotechnology and National Center for Maize Improvement, China Agricultural University, 100094, Beijing, China aut NATIONALLICENCE 700 1- Wang Guoying State Key Laboratory of Agrobiotechnology and National Center for Maize Improvement, China Agricultural University, 100094, Beijing, China aut NATIONALLICENCE 773 0- Plant Cell, Tissue and Organ Culture Springer Netherlands 88/1(2007-01-01), 11-20 0167-6857 88:1<11 2007 88 11240 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer