caa a22 4500 463235630 CHVBK 20180405153256.0 cr unu---uuuuu 170326e20070101xx s 000 0 eng 10.1007/s11240-006-9173-4 doi (NATIONALLICENCE)springer-10.1007/s11240-006-9173-4 Increasing maize seed weight by enhancing the cytoplasmic ADP-glucose pyrophosphorylase activity in transgenic maize plants [Elektronische Daten] [Zhangying Wang, Xiaoping Chen, Jianhua Wang, Tingsong Liu, Yan Liu, Li Zhao, Guoying Wang] ADP-glucose pyrophosphorylase (AGPase) plays a key role in regulating starch biosynthesis in cereal seeds and is likely the most important determinant of seed strength. The Escherichia coli mutant glgC gene (glgC16), which encodes a highly active and allosterically insensitive AGPase, was introduced into maize (Zea mays L.) under the control of an endosperm-specific promoter. Developing seeds from transgenic maize plants showed up to 2-4-fold higher levels of AGPase activity in the presence of 5mM inorganic phosphate (Pi). Transgenic plants with higher cytoplasmic AGPase activity under Pi-inhibitory conditions showed increases (13-25%) in seed weight over the untransformed control. In addition, in all transgenic maize plants, the seeds were fully filled, and the seed number of transgenic plants had no significant difference compared with that of untransformed control. These results indicate that increasing cytoplasmic AGPase activity has a marked effect on sink activity and, in turn, seed weight in transgenic maize plants. Springer Science+Business Media B.V., 2006 glgC16 nationallicence Seed weight nationallicence Sink strength nationallicence Starch biosynthesis nationallicence Zeamays L nationallicence AGPase : ADP-glucose pyrophosphorylase nationallicence Bt2 : Brittle2 nationallicence DAP : Days after self-pollination nationallicence 3-PGA : 3-phosphoglyceric acid nationallicence Pi : Inorganic phosphate nationallicence Sh2 : Shrunken-2 nationallicence Wang Zhangying State Key Laboratory of Agrobiotechnology, China Agricultural University, 100094, Beijing, China aut Chen Xiaoping State Key Laboratory of Agrobiotechnology, China Agricultural University, 100094, Beijing, China aut Wang Jianhua State Key Laboratory of Agrobiotechnology, China Agricultural University, 100094, Beijing, China aut Liu Tingsong State Key Laboratory of Agrobiotechnology, China Agricultural University, 100094, Beijing, China aut Liu Yan State Key Laboratory of Agrobiotechnology, China Agricultural University, 100094, Beijing, China aut Zhao Li State Key Laboratory of Agrobiotechnology, China Agricultural University, 100094, Beijing, China aut Wang Guoying State Key Laboratory of Agrobiotechnology, China Agricultural University, 100094, Beijing, China aut Plant Cell, Tissue and Organ Culture Springer Netherlands 88/1(2007-01-01), 83-92 0167-6857 88:1<83 2007 88 11240 https://doi.org/10.1007/s11240-006-9173-4 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11240-006-9173-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Wang Zhangying State Key Laboratory of Agrobiotechnology, China Agricultural University, 100094, Beijing, China aut NATIONALLICENCE 700 1- Chen Xiaoping State Key Laboratory of Agrobiotechnology, China Agricultural University, 100094, Beijing, China aut NATIONALLICENCE 700 1- Wang Jianhua State Key Laboratory of Agrobiotechnology, China Agricultural University, 100094, Beijing, China aut NATIONALLICENCE 700 1- Liu Tingsong State Key Laboratory of Agrobiotechnology, China Agricultural University, 100094, Beijing, China aut NATIONALLICENCE 700 1- Liu Yan State Key Laboratory of Agrobiotechnology, China Agricultural University, 100094, Beijing, China aut NATIONALLICENCE 700 1- Zhao Li State Key Laboratory of Agrobiotechnology, China Agricultural University, 100094, Beijing, China aut NATIONALLICENCE 700 1- Wang Guoying State Key Laboratory of Agrobiotechnology, China Agricultural University, 100094, Beijing, China aut NATIONALLICENCE 773 0- Plant Cell, Tissue and Organ Culture Springer Netherlands 88/1(2007-01-01), 83-92 0167-6857 88:1<83 2007 88 11240 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer