caa a22 4500 463235649 CHVBK 20180405153256.0 cr unu---uuuuu 170326e20070101xx s 000 0 eng 10.1007/s11240-006-9185-0 doi (NATIONALLICENCE)springer-10.1007/s11240-006-9185-0 Preliminary research on conversion of encapsulated somatic embryos of Citrus reticulata Blanco, cv. Mandarino Tardivo di Ciaculli [Elektronische Daten] [Germanà Antonietta, Hafiz Ahmad, Micheli Maurizio, Standardi Alvaro] Somatic embryogenesis was obtained through anther culture of Citrus reticulata, cv. Mandarino Tardivo di Ciaculli. The work was carried out to evaluate the response of somatic embryos inside a sodium alginate coating to different storage periods, and to the effects of the germicide PPM (1mll−1) and the fungicide Thiophanate-methyl (100mgl−1). The effect of these alone or in combination, added to the artificial endosperm on the performance of the encapsulated somatic embryos was tested. After 45days of culturing, sprouting, root development and conversion were recorded. The encapsulated somatic embryos can be conveniently stored at 4°C, but not for a period longer than 60days. The presence of Thiophanate-methyl in the artificial endosperm resulted in high levels of sprouting with promising frequencies of root development and conversion in to plantlets under invitro and ex vitro conditions. The concentration of PPM used, alone or in combination with the fungicide, showed some depressing effects in this regard. The prelimnary results obtained with the application of Thiophanate-methyl in a nutrient enriched alginate matrix are promising and warrant further experimentation in attempts to find a solution for the problems faced with ex vitro use of encapsulated somatic embryos. Springer Science+Business Media B.V., 2006 Cold storage embryogenesis nationallicence Fungicide nationallicence Sodium alginate coating nationallicence Synthetic seed nationallicence MTC : cv.Mandarino Tardivo di Ciaculli nationallicence PPM : Plant preservative mixture nationallicence SE : Somatic embryo nationallicence ESE : Encapsulated somatic embryo nationallicence TM : Thiophanate-methyl nationallicence Antonietta Germanà SENFIMIZO Department, University of Palermo, Viale delle Scienze, 11, 90128, Palermo, Italy aut Ahmad Hafiz Department of Horticulture, University of Arid Agriculture, Murree Road, Rawalpindi, Pakistan aut Maurizio Micheli Department of Agricultural and Environmental Sciences, University of Perugia, Borgo XX Giugno, 74, 06121, Perugia, Italy aut Alvaro Standardi Department of Agricultural and Environmental Sciences, University of Perugia, Borgo XX Giugno, 74, 06121, Perugia, Italy aut Plant Cell, Tissue and Organ Culture Springer Netherlands 88/1(2007-01-01), 117-120 0167-6857 88:1<117 2007 88 11240 https://doi.org/10.1007/s11240-006-9185-0 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11240-006-9185-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Antonietta Germanà SENFIMIZO Department, University of Palermo, Viale delle Scienze, 11, 90128, Palermo, Italy aut NATIONALLICENCE 700 1- Ahmad Hafiz Department of Horticulture, University of Arid Agriculture, Murree Road, Rawalpindi, Pakistan aut NATIONALLICENCE 700 1- Maurizio Micheli Department of Agricultural and Environmental Sciences, University of Perugia, Borgo XX Giugno, 74, 06121, Perugia, Italy aut NATIONALLICENCE 700 1- Alvaro Standardi Department of Agricultural and Environmental Sciences, University of Perugia, Borgo XX Giugno, 74, 06121, Perugia, Italy aut NATIONALLICENCE 773 0- Plant Cell, Tissue and Organ Culture Springer Netherlands 88/1(2007-01-01), 117-120 0167-6857 88:1<117 2007 88 11240 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer