caa a22 4500 463235665 CHVBK 20180405153256.0 cr unu---uuuuu 170326e20070101xx s 000 0 eng 10.1007/s11240-006-9172-5 doi (NATIONALLICENCE)springer-10.1007/s11240-006-9172-5 Node-derived cultures with high-morphogenic competence in barley and wheat [Elektronische Daten] [Vijendra Sharma, Robert Hänsch, Ralf Mendel, Jutta Schulze] A successful regeneration system is presented for elite cultivars in barley and wheat based on nodes. Nodal explants were excised from in vitro and ex vitro grown plants. A combination of 8.28μM 4-amino-3,5,6-trichloropicolinic acid and 4.54μM to 22.71μm thidiazuron (TDZ) used in MS-based medium containing 60gl−1 maltose favoured induction of clumps of multiple shoots/buds with or without callus formation in the primary cultures. Within 8-10weeks upon further subcultures, the proliferation into callus with rapid and continuously forming adventitious buds containing clusters of meristemoids, termed meristematic bulk tissue (MBT) was obtained. Lowering the levels of growth regulators resulted in redifferentiation of shoots, which elongated, rooted, developed into morphologically normal plants and set seeds normally. With a frequency ranging between 37 and 82% the nodes raised from in vitro grown plants were proliferated into MBT independent of TDZ concentration, cultivar and species. The average number of shoots per responding node in different cultivars was 7-15 in barley and 1-6 in wheat after 12-14weeks. Nodes from greenhouse grown plants mainly responded for callus formation with poor development of MBT. Springer Science+Business Media B.V., 2006 Meristemoids nationallicence Meristematic bulk tissue nationallicence Nodal shoot segment nationallicence Picloram nationallicence Shoot morphogenesis nationallicence Thidiazuron nationallicence IBA : Indole-3-butyric acid nationallicence MBT : Meristematic bulk tissue nationallicence MS : Murashige and Skoog nationallicence NSS : Nodal shoot segment nationallicence Picloram : 4-amino-3,5,6-trichloropicolinic acid nationallicence TDZ : Thidiazuron nationallicence Sharma Vijendra Department of Plant Biology, Technical University of Braunschweig, Humboldtstr. 1, D-38106, Braunschweig, Germany aut Hänsch Robert Department of Plant Biology, Technical University of Braunschweig, Humboldtstr. 1, D-38106, Braunschweig, Germany aut Mendel Ralf Department of Plant Biology, Technical University of Braunschweig, Humboldtstr. 1, D-38106, Braunschweig, Germany aut Schulze Jutta Department of Plant Biology, Technical University of Braunschweig, Humboldtstr. 1, D-38106, Braunschweig, Germany aut Plant Cell, Tissue and Organ Culture Springer Netherlands 88/1(2007-01-01), 21-33 0167-6857 88:1<21 2007 88 11240 https://doi.org/10.1007/s11240-006-9172-5 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11240-006-9172-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Sharma Vijendra Department of Plant Biology, Technical University of Braunschweig, Humboldtstr. 1, D-38106, Braunschweig, Germany aut NATIONALLICENCE 700 1- Hänsch Robert Department of Plant Biology, Technical University of Braunschweig, Humboldtstr. 1, D-38106, Braunschweig, Germany aut NATIONALLICENCE 700 1- Mendel Ralf Department of Plant Biology, Technical University of Braunschweig, Humboldtstr. 1, D-38106, Braunschweig, Germany aut NATIONALLICENCE 700 1- Schulze Jutta Department of Plant Biology, Technical University of Braunschweig, Humboldtstr. 1, D-38106, Braunschweig, Germany aut NATIONALLICENCE 773 0- Plant Cell, Tissue and Organ Culture Springer Netherlands 88/1(2007-01-01), 21-33 0167-6857 88:1<21 2007 88 11240 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer