caa a22 4500 463235681 CHVBK 20180405153256.0 cr unu---uuuuu 170326e20070301xx s 000 0 eng 10.1007/s11240-006-9200-5 doi (NATIONALLICENCE)springer-10.1007/s11240-006-9200-5 Regeneration and genetic transformation of Hagenia abyssinica (Bruce) J.F. Gmel. (Rosaceae) with rolB gene [Elektronische Daten] [Tileye Feyissa, Li-Hua Zhu, Legesse Negash, Margareta Welander] The effects of growth regulators, wounding and antibiotics on regeneration of Hagenia abyssinica were investigated and the rolB gene was introduced into this species by Agrobacterium-mediated transformation. Regeneration was affected by type of growth regulators, wounding and antibiotics. Up to 100% regeneration could be obtained. Three transformed clones (T1, T2.1, T2.2), confirmed by PCR and Southern blot, were obtained only by excluding kanamycin from the selection medium 6weeks after culture, followed by selection during shoot multiplication. RT-PCR revealed strong expression of rolB gene in shoots and roots of all the transgenic clones, but from leaf samples, it was detected only in T1. Rooting frequency was 77% (T1), 50% (T2.1), 57% (T2.2) and 0% for control shoots on growth regulator-free rooting medium. Springer Science+Business Media B.V., 2007 Agrobacterium -mediated transformation nationallicence Antibiotic nationallicence Growth regulator nationallicence Rooting nationallicence Wounding nationallicence 2,4-D : 2,4-dichlorophenoxyacetic acid nationallicence BAP : 6-benzylaminopurine nationallicence IAA : indole-3-acetic acid nationallicence IBA : indole-3-butyric acid nationallicence MS : Murashige and Skoog nationallicence NAA : α-naphthaleneacetic acid nationallicence nptII : neomycinphosphotransferase II nationallicence rolB : root locus B nationallicence Feyissa Tileye Department of Crop Science, Swedish University of Agricultural Sciences, P.O. Box 44, 230 53, Alnarp, Sweden aut Zhu Li-Hua Department of Crop Science, Swedish University of Agricultural Sciences, P.O. Box 44, 230 53, Alnarp, Sweden aut Negash Legesse Department of Biology, Faculty of Science, Addis Ababa University, P.O. Box 1176, Addis Ababa, Ethiopia aut Welander Margareta Department of Crop Science, Swedish University of Agricultural Sciences, P.O. Box 44, 230 53, Alnarp, Sweden aut Plant Cell, Tissue and Organ Culture Kluwer Academic Publishers 88/3(2007-03-01), 277-288 0167-6857 88:3<277 2007 88 11240 https://doi.org/10.1007/s11240-006-9200-5 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11240-006-9200-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Feyissa Tileye Department of Crop Science, Swedish University of Agricultural Sciences, P.O. Box 44, 230 53, Alnarp, Sweden aut NATIONALLICENCE 700 1- Zhu Li-Hua Department of Crop Science, Swedish University of Agricultural Sciences, P.O. Box 44, 230 53, Alnarp, Sweden aut NATIONALLICENCE 700 1- Negash Legesse Department of Biology, Faculty of Science, Addis Ababa University, P.O. Box 1176, Addis Ababa, Ethiopia aut NATIONALLICENCE 700 1- Welander Margareta Department of Crop Science, Swedish University of Agricultural Sciences, P.O. Box 44, 230 53, Alnarp, Sweden aut NATIONALLICENCE 773 0- Plant Cell, Tissue and Organ Culture Kluwer Academic Publishers 88/3(2007-03-01), 277-288 0167-6857 88:3<277 2007 88 11240 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer