caa a22 4500 463235703 CHVBK 20180405153256.0 cr unu---uuuuu 170326e20070301xx s 000 0 eng 10.1007/s11240-006-9198-8 doi (NATIONALLICENCE)springer-10.1007/s11240-006-9198-8 Embryo abortion and histological features in the interspecific cross between Phaseolus vulgaris L. and P. coccineus L [Elektronische Daten] [Pamphile Nguema Ndoutoumou, André Toussaint, Jean Baudoin] In this paper, the causes of early embryo abortion in the reciprocal crosses between Phaseolus vulgaris L. (a cultivar) and Phaseolus coccineus L. (a wild form) were studied. Methacrylate resin sections, 3-5μm thick, of 3 to 14day-old seeds were used to examine the embryo developmental stages and the state of seed tissue. It was observed that, embryos aborted at different developmental stages (globular to early cotyledon) depending on the maternal parent. The use of P. coccineus cytoplasm resulted in a higher number of abortion than in reciprocal crosses. Many of them took place between 5 and 6days after pollination (DAP). Histological analyses permitted to observe that the embryo development was slower in the cross between P. coccineus and P. vulgaris, compared to parental seeds. It would be related to a deficient endosperm development in reciprocal crosses and, in some extent, hypertrophy of the suspensor might be the main cause of early embryo abortion. Then, it would be practical to overcome this incompatibility by rescuing the embryo at the globular stage of development. Springer Science+Business Media B.V., 2007 Embryogenesis nationallicence Histology nationallicence Hybridization nationallicence Incompatibility barriers nationallicence Suspensor nationallicence DAP : Days after pollination nationallicence HEMA : 2-Hydroxyethyl Methacrylate nationallicence Nguema Ndoutoumou Pamphile Faculté universitaire des Sciences agronomiques de Gembloux - Unité de Phytotechnie Tropicale et Horticulture, Passage des Déportés, 2, 5030, Gembloux, Belgium aut Toussaint André Faculté universitaire des Sciences agronomiques de Gembloux - Unité de Phytotechnie Tropicale et Horticulture, Passage des Déportés, 2, 5030, Gembloux, Belgium aut Baudoin Jean Faculté universitaire des Sciences agronomiques de Gembloux - Unité de Phytotechnie Tropicale et Horticulture, Passage des Déportés, 2, 5030, Gembloux, Belgium aut Plant Cell, Tissue and Organ Culture Kluwer Academic Publishers 88/3(2007-03-01), 329-332 0167-6857 88:3<329 2007 88 11240 https://doi.org/10.1007/s11240-006-9198-8 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11240-006-9198-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Nguema Ndoutoumou Pamphile Faculté universitaire des Sciences agronomiques de Gembloux - Unité de Phytotechnie Tropicale et Horticulture, Passage des Déportés, 2, 5030, Gembloux, Belgium aut NATIONALLICENCE 700 1- Toussaint André Faculté universitaire des Sciences agronomiques de Gembloux - Unité de Phytotechnie Tropicale et Horticulture, Passage des Déportés, 2, 5030, Gembloux, Belgium aut NATIONALLICENCE 700 1- Baudoin Jean Faculté universitaire des Sciences agronomiques de Gembloux - Unité de Phytotechnie Tropicale et Horticulture, Passage des Déportés, 2, 5030, Gembloux, Belgium aut NATIONALLICENCE 773 0- Plant Cell, Tissue and Organ Culture Kluwer Academic Publishers 88/3(2007-03-01), 329-332 0167-6857 88:3<329 2007 88 11240 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer