caa a22 4500 463235711 CHVBK 20180405153256.0 cr unu---uuuuu 170326e20070301xx s 000 0 eng 10.1007/s11240-006-9189-9 doi (NATIONALLICENCE)springer-10.1007/s11240-006-9189-9 Effect of radiation on regeneration of Chinese narcissus and analysis of genetic variation with AFLP and RAPD markers [Elektronische Daten] [Gang Lu, Xiaoying Zhang, Yijing Zou, Qingcheng Zou, Xun Xiang, Jiashu Cao] The aim of our study was to establish an efficient in vitro propagation protocol for Chinese narcissus (Narcissus tazetta var. chinensis) to obtain variants of this species using γ-radiation treatment and evaluate the effectiveness of this system for variant induction using amplification fragment length polymorphism (AFLP) and randomly amplified polymorphic DNA (RAPD) analysis. Various doses (5-100Gy) of gamma rays were applied to investigate the effect of radiation on adventitious bud formation from bulb-scales and the survival rate of plantlets. It was demonstrated that the regeneration of Chinese narcissus was very sensitive to gamma radiation even at low doses. The survival and multiplication rate significantly decreased with an increase of radiation dose. The optimal irradiation dose for survival and mutation induction was approximately 10Gy. The genetic variations among the regenerants derived from irradiated explants were evaluated by DNA fingerprinting using RAPD and AFLP markers which detected a variation frequency of 8.33% and 15.48% respectively. The high frequency of mutants detected by molecular markers indicated that treatment of in vitro cultures with γ-rays may be an effective way to improve narcissus cultivars. Springer Science+Business Media B.V., 2007 Narcissus tazetta var. chinensis nationallicence Irradiation nationallicence In vitro culture nationallicence Mutants nationallicence Molecular marker nationallicence AFLP : Amplification fragment length polymorphism nationallicence BA : 6-Benzylaminopurine nationallicence IBA : Indolebutyric acid nationallicence DTT : Dithiothreitol nationallicence NAA : α-Naphthaleneacetic acid nationallicence RAPD : Random amplified polymorphic DNA nationallicence Lu Gang Department of Horticulture, Zhejiang University, 268 Kuaixuan Road, 310029, Hangzhou, Zhejiang, P.R. China aut Zhang Xiaoying Department of Horticulture, Zhejiang University, 268 Kuaixuan Road, 310029, Hangzhou, Zhejiang, P.R. China aut Zou Yijing Department of Horticulture, Zhejiang University, 268 Kuaixuan Road, 310029, Hangzhou, Zhejiang, P.R. China aut Zou Qingcheng Department of Horticulture, Zhejiang University, 268 Kuaixuan Road, 310029, Hangzhou, Zhejiang, P.R. China aut Xiang Xun Department of Horticulture, Zhejiang University, 268 Kuaixuan Road, 310029, Hangzhou, Zhejiang, P.R. China aut Cao Jiashu Department of Horticulture, Zhejiang University, 268 Kuaixuan Road, 310029, Hangzhou, Zhejiang, P.R. China aut Plant Cell, Tissue and Organ Culture Kluwer Academic Publishers 88/3(2007-03-01), 319-327 0167-6857 88:3<319 2007 88 11240 https://doi.org/10.1007/s11240-006-9189-9 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11240-006-9189-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Lu Gang Department of Horticulture, Zhejiang University, 268 Kuaixuan Road, 310029, Hangzhou, Zhejiang, P.R. China aut NATIONALLICENCE 700 1- Zhang Xiaoying Department of Horticulture, Zhejiang University, 268 Kuaixuan Road, 310029, Hangzhou, Zhejiang, P.R. China aut NATIONALLICENCE 700 1- Zou Yijing Department of Horticulture, Zhejiang University, 268 Kuaixuan Road, 310029, Hangzhou, Zhejiang, P.R. China aut NATIONALLICENCE 700 1- Zou Qingcheng Department of Horticulture, Zhejiang University, 268 Kuaixuan Road, 310029, Hangzhou, Zhejiang, P.R. China aut NATIONALLICENCE 700 1- Xiang Xun Department of Horticulture, Zhejiang University, 268 Kuaixuan Road, 310029, Hangzhou, Zhejiang, P.R. China aut NATIONALLICENCE 700 1- Cao Jiashu Department of Horticulture, Zhejiang University, 268 Kuaixuan Road, 310029, Hangzhou, Zhejiang, P.R. China aut NATIONALLICENCE 773 0- Plant Cell, Tissue and Organ Culture Kluwer Academic Publishers 88/3(2007-03-01), 319-327 0167-6857 88:3<319 2007 88 11240 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer