caa a22 4500 463235738 CHVBK 20180405153256.0 cr unu---uuuuu 170326e20070301xx s 000 0 eng 10.1007/s11240-007-9202-y doi (NATIONALLICENCE)springer-10.1007/s11240-007-9202-y Enhancement of somatic embryogenesis and plant regeneration in Japanese larch ( Larix leptolepis ) [Elektronische Daten] [Yong Kim, Heung Moon] Different nitrogen sources, abscisic acid (ABA), gellan gum at various concentrations, and osmotica were evaluated for their effects on maturation of somatic embryo (SE) in Japanese larch (Larix leptolepis). Different concentrations of l-glutamine or casein hydrolysate (CH) in the medium were also compared. The highest number of matured embryos was obtained with ½Litvay (LM) medium supplemented with 1.71mM l-glutamine and 250mgl−1 CH. In terms of osmoticum effect, the highest number of cotyledonary SEs was produced in medium containing 0.2M maltose. As for the effects of ABA and gellan gum concentration, the highest number of cotyledonary SEs was achieved on a medium containing 60μM ABA and 0.8% gellan gum. In addition, the best plantlet conversion frequency (35.5%) was obtained with SEs derived from the treatment with 60μM ABA and 0.8% gellan gum. Springer Science+Business Media B.V., 2007 Abscisic acid nationallicence Casein hydrolysate nationallicence Maltose nationallicence Micropropagation nationallicence Germination nationallicence ABA : Abscisic acid nationallicence CH : Casein hydrolysate nationallicence ESM : Embryogenic suspensor mass nationallicence LM : Litvay (1985) medium nationallicence PEG : Polyethyleneglycol nationallicence SE : Somatic embryo nationallicence Kim Yong Division of Biotechnology, Forest Genetics Research Institute, P.O. Box 24, 441-350, Suwon, Kyonggido, Republic of Korea aut Moon Heung Division of Biotechnology, Forest Genetics Research Institute, P.O. Box 24, 441-350, Suwon, Kyonggido, Republic of Korea aut Plant Cell, Tissue and Organ Culture Kluwer Academic Publishers 88/3(2007-03-01), 241-245 0167-6857 88:3<241 2007 88 11240 https://doi.org/10.1007/s11240-007-9202-y text/html Onlinezugriff via DOI 1 brief-communication jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11240-007-9202-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kim Yong Division of Biotechnology, Forest Genetics Research Institute, P.O. Box 24, 441-350, Suwon, Kyonggido, Republic of Korea aut NATIONALLICENCE 700 1- Moon Heung Division of Biotechnology, Forest Genetics Research Institute, P.O. Box 24, 441-350, Suwon, Kyonggido, Republic of Korea aut NATIONALLICENCE 773 0- Plant Cell, Tissue and Organ Culture Kluwer Academic Publishers 88/3(2007-03-01), 241-245 0167-6857 88:3<241 2007 88 11240 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer