caa a22 4500 463235746 CHVBK 20180405153256.0 cr unu---uuuuu 170326e20070301xx s 000 0 eng 10.1007/s11240-006-9197-9 doi (NATIONALLICENCE)springer-10.1007/s11240-006-9197-9 Classen Birgit Institute of Pharmacy, Department of Pharmaceutical Biology, University of Kiel, Gutenbergstr. 76, 24118, Kiel, Germany aut Characterization of an arabinogalactan-protein from suspension culture of Echinacea purpurea [Elektronische Daten] [Birgit Classen] Suspension cultures of Echinacea purpurea have been established in MS medium supplemented with 2,4-D and an arabinogalactan-protein (AGP) was purified from the secreted soluble polymers by precipitation with ethanol, followed by precipitation with β-glucosyl Yariv reagent. It revealed typical features of AGPs: a high amount of polysaccharide (90%w/w) with the dominating monosaccharides galactose and arabinose and some glucuronic acid, and a small protein moiety (10%w/w) with the main amino acids Ala, Hyp, Glx, Ser, Asx and Thr. Linkage- and NMR-analyses showed the polysaccharide part to be composed of a branched core-polysaccharide of 3-, 6- and 3,6-linked Galp residues with terminal Araf, Arap, Galp and GlcAp residues. Compared to an AGP from pressed juice of the aerial parts of Echinacea purpurea, differences particularly in terminal arabinose mono- and oligosaccharides in arabinogalactan (AG) side branches could be detected. Testing of different AGP-antibodies with both AGPs confirmed the results of the analytical investigations. Binding of AGPs from plant and cell cultures to LM2, a monoclonal AGP-antibody reacting with a GlcA containing epitope, was comparable. The reactivity of a monoclonal antibody raised against the AGP from the plant recognizing a galactan epitope was also nearly similar with both AGPs. In contrast, polyclonal antibodies raised against the AGP from the plant and directed against an Araf-containing epitope of the AG side branches showed nearly no cross reactivity with the AGP from cell culture. Springer Science+Business Media B.V., 2007 Arabinogalactan-protein nationallicence Echinacea purpurea nationallicence Suspension culture nationallicence β-glucosyl Yariv reagent nationallicence AGP-antibody nationallicence AG : Arabinogalactan nationallicence AGP : Arabinogalactan-protein nationallicence 2,4-D : 2,4-Dichlorophenoxyacetic acid nationallicence GLC-MS : Gas liquid chromatography-mass spectrometry nationallicence MS : Murashige and Skoog medium (1962) nationallicence Plant Cell, Tissue and Organ Culture Kluwer Academic Publishers 88/3(2007-03-01), 267-275 0167-6857 88:3<267 2007 88 11240 https://doi.org/10.1007/s11240-006-9197-9 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11240-006-9197-9 text/html Onlinezugriff via DOI NATIONALLICENCE 100 1- Classen Birgit Institute of Pharmacy, Department of Pharmaceutical Biology, University of Kiel, Gutenbergstr. 76, 24118, Kiel, Germany aut NATIONALLICENCE 773 0- Plant Cell, Tissue and Organ Culture Kluwer Academic Publishers 88/3(2007-03-01), 267-275 0167-6857 88:3<267 2007 88 11240 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer