caa a22 4500 463235762 CHVBK 20180405153256.0 cr unu---uuuuu 170326e20070301xx s 000 0 eng 10.1007/s11240-006-9174-3 doi (NATIONALLICENCE)springer-10.1007/s11240-006-9174-3 Benzyladenine and low temperature promote phase transition from juvenile to vegetative adult in bulblets of Lilium × formolongi ‘White Aga' cultured in vitro [Elektronische Daten] [Takateru Ishimori, Yoshiji Niimi, Dong-Sheng Han] Scales excised from lily bulblets were cultured on MS medium supplemented with 0.044 or 4.4μM BA in the dark for 180days. The culture period was divided into stage 1 (day 0-30), stage 2 (day 31-90) and stage 3 (day 91-180). The scales were cultured at 25°C in stage 1, 25°C or 8°C in stage 2, and 25°C in stage 3. When the scales were cultured on medium with 4.4μM BA at 25°C for 180days, bulblets with and without an elongated stem were produced. The percentage of bulblets with elongated stems greatly increased when the scales had been cultured at 8°C in stage 2. On medium with 0.044μM BA, only bulblets without elongated stems were produced. The diameter of shoot primodia significantly enlarged in bulblets produced on medium with 4.4μM BA at 8°C in stage 2 and no such enlargement occurred under the other conditions. Nearly square parenchyma cells were observed in the non-elongated shoot primodia in the former bulblets but not in the latter. These cells changed into longitudinally rectangular ones in the internode of elongated stems. Procambium was arranged almost parallel to the shoot axis in the stem of bulblets in the medium with 4.4μM BA, but not in the medium with 0.044μM BA. Springer Science+Business Media B.V., 2007 Cell expansion nationallicence Procambium nationallicence Rectangular parenchyma cell nationallicence Stem elongation nationallicence BA : N6-benzyladenine nationallicence FAA : formalin/acetic acid/ethanol nationallicence MS : Murashige and Skoog (Murashige and Skoog 1962) nationallicence NAA : 1-naphthaleneacetic acid nationallicence Ishimori Takateru Faculty of Agriculture, Niigata University, 2-8050 Ikarashi, 950-2181, Niigata, Japan aut Niimi Yoshiji Faculty of Agriculture, Niigata University, 2-8050 Ikarashi, 950-2181, Niigata, Japan aut Han Dong-Sheng Faculty of Agriculture, Niigata University, 2-8050 Ikarashi, 950-2181, Niigata, Japan aut Plant Cell, Tissue and Organ Culture Kluwer Academic Publishers 88/3(2007-03-01), 313-318 0167-6857 88:3<313 2007 88 11240 https://doi.org/10.1007/s11240-006-9174-3 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11240-006-9174-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ishimori Takateru Faculty of Agriculture, Niigata University, 2-8050 Ikarashi, 950-2181, Niigata, Japan aut NATIONALLICENCE 700 1- Niimi Yoshiji Faculty of Agriculture, Niigata University, 2-8050 Ikarashi, 950-2181, Niigata, Japan aut NATIONALLICENCE 700 1- Han Dong-Sheng Faculty of Agriculture, Niigata University, 2-8050 Ikarashi, 950-2181, Niigata, Japan aut NATIONALLICENCE 773 0- Plant Cell, Tissue and Organ Culture Kluwer Academic Publishers 88/3(2007-03-01), 313-318 0167-6857 88:3<313 2007 88 11240 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer