caa a22 4500 463235770 CHVBK 20180405153256.0 cr unu---uuuuu 170326e20070301xx s 000 0 eng 10.1007/s11240-007-9201-z doi (NATIONALLICENCE)springer-10.1007/s11240-007-9201-z Interspecific hybridization of Prunus persica with P. armeniaca and P. salicina using embryo rescue [Elektronische Daten] [Wen Liu, Xuesen Chen, Guanjun Liu, Qing Liang, Tianming He, Jianrong Feng] Embryo rescue technique was used successfully to produce interspecific hybrids by crossing peach (P. persica) as a female parent with apricot (P. armeniaca) and plum (P. salicica). In those crosses that had ‘Yuhualu' or ‘Zhonghuashoutao' as female parents, hybrid embryos aborted from the 7th or 8th week after pollination mainly due to post-pollination incompatibility. An embryo rescue protocol was established to rescue such embryos and recover hybrid plants. Modified half-strength MS medium containing 4mgl−1 6-BA and 0.5mgl−1 IBA produced up to 90% germination in the embryos. Modified MS medium with 1.0mgl−1 6-BA and 1.0mgl−1 IBA gave the highest bud induction and multiplication whereas modified MS medium containing 0.5mgl−1 IAA and 0.2mgl−1 NAA gave the best rooting percentage. All the hybrids obtained using this embryo rescue technique were verified using simple sequence repeat (SSR) markers. A series of pollen treatments were carried out to partially overcome pre-pollination incompatibility, and it was found accidentally that pollen treatment with electrostatic field not only improved pollen germination but also increased the multiplication coefficient of embryo-induced shoots. Springer Science+Business Media B.V., 2007 Prunus nationallicence Interspecific hybridization nationallicence Embryo rescue nationallicence IAA : indole-3-acetic acid nationallicence IBA : indole-3-butyric acid nationallicence NAA : α-naphthaleneacetic acid nationallicence 6-BA : 6-benzyladenine nationallicence MS : Murashige and Skoog (1962) medium nationallicence PF : embryo length/seed length nationallicence SSR : simple sequence repeat nationallicence Liu Wen Fruit Biology Laboratory, College of Horticulture Science and Engineering, Shandong Agricultural University, 271018, Shandong, PR China aut Chen Xuesen Fruit Biology Laboratory, College of Horticulture Science and Engineering, Shandong Agricultural University, 271018, Shandong, PR China aut Liu Guanjun College of Food Science and Engineering, Shandong Agricultural University, 271018, Shandong, PR China aut Liang Qing Fruit Biology Laboratory, College of Horticulture Science and Engineering, Shandong Agricultural University, 271018, Shandong, PR China aut He Tianming Fruit Biology Laboratory, College of Horticulture Science and Engineering, Shandong Agricultural University, 271018, Shandong, PR China aut Feng Jianrong Fruit Biology Laboratory, College of Horticulture Science and Engineering, Shandong Agricultural University, 271018, Shandong, PR China aut Plant Cell, Tissue and Organ Culture Kluwer Academic Publishers 88/3(2007-03-01), 289-299 0167-6857 88:3<289 2007 88 11240 https://doi.org/10.1007/s11240-007-9201-z text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11240-007-9201-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Liu Wen Fruit Biology Laboratory, College of Horticulture Science and Engineering, Shandong Agricultural University, 271018, Shandong, PR China aut NATIONALLICENCE 700 1- Chen Xuesen Fruit Biology Laboratory, College of Horticulture Science and Engineering, Shandong Agricultural University, 271018, Shandong, PR China aut NATIONALLICENCE 700 1- Liu Guanjun College of Food Science and Engineering, Shandong Agricultural University, 271018, Shandong, PR China aut NATIONALLICENCE 700 1- Liang Qing Fruit Biology Laboratory, College of Horticulture Science and Engineering, Shandong Agricultural University, 271018, Shandong, PR China aut NATIONALLICENCE 700 1- He Tianming Fruit Biology Laboratory, College of Horticulture Science and Engineering, Shandong Agricultural University, 271018, Shandong, PR China aut NATIONALLICENCE 700 1- Feng Jianrong Fruit Biology Laboratory, College of Horticulture Science and Engineering, Shandong Agricultural University, 271018, Shandong, PR China aut NATIONALLICENCE 773 0- Plant Cell, Tissue and Organ Culture Kluwer Academic Publishers 88/3(2007-03-01), 289-299 0167-6857 88:3<289 2007 88 11240 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer