caa a22 4500 463235789 CHVBK 20180405153256.0 cr unu---uuuuu 170326e20070301xx s 000 0 eng 10.1007/s11240-006-9196-x doi (NATIONALLICENCE)springer-10.1007/s11240-006-9196-x Plant regeneration from leaf protoplasts of Solanum virginianum L. ( Solanaceae ) [Elektronische Daten] [L. Borgato, F. Pisani, A. Furini] Leaves of Solanum virginianum plants were used for protoplast isolation. To support cell wall formation and cell division, protoplasts were cultured in thin alginate layers floated in liquid medium. When protoplasts were plated at a density of 1.0×106/ml in Kao and Michyaluk (KMp8) medium supplemented with 0.5mg/l zeatin, 1.0mg/l 2,4-dichlorophenoxyacetic acid, and 1.0mg/l α-naphthaleneacetic acid, 42.3% of the dividing cells developed microcalli in 3-4weeks. Shoot formation via organogenesis of protoplast-derived calli was achieved for 28% of calli transferred to solidified KMp8 medium supplemented with 2.0g/l zeatin and 0.1mg/l 3-indol acetic acid in about 2weeks. Further shoot development was observed in Murashige and Skoog (MS) medium without growth regulators and roots were induced after transfer to MS medium containing 1.0mg/l 3-indol butyric acid. Regenerated plants have normal morphology. Springer Science+Business Media B.V., 2007 Organogenesis nationallicence Plant regeneration nationallicence Protoplast culture nationallicence Solanum virginianum nationallicence MS : Murashige and Skoog medium nationallicence KMp8 : Kao and Michyaluk medium nationallicence PVP : Polyvinylpirrolidone nationallicence IAA : 3-indol acetic acid nationallicence NAA : α-naphthaleneacetic acid nationallicence IBA : 3-indol butyric acid nationallicence 2,4- d : 2,4-dichlorophenoxyacetic acid nationallicence Kin : Kinetin nationallicence Borgato L. Department of Science and Technology, University of Verona, Strada le Grazie 15, 37134, Verona, Italy aut Pisani F. Department of Science and Technology, University of Verona, Strada le Grazie 15, 37134, Verona, Italy aut Furini A. Department of Science and Technology, University of Verona, Strada le Grazie 15, 37134, Verona, Italy aut Plant Cell, Tissue and Organ Culture Kluwer Academic Publishers 88/3(2007-03-01), 247-252 0167-6857 88:3<247 2007 88 11240 https://doi.org/10.1007/s11240-006-9196-x text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11240-006-9196-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Borgato L. Department of Science and Technology, University of Verona, Strada le Grazie 15, 37134, Verona, Italy aut NATIONALLICENCE 700 1- Pisani F. Department of Science and Technology, University of Verona, Strada le Grazie 15, 37134, Verona, Italy aut NATIONALLICENCE 700 1- Furini A. Department of Science and Technology, University of Verona, Strada le Grazie 15, 37134, Verona, Italy aut NATIONALLICENCE 773 0- Plant Cell, Tissue and Organ Culture Kluwer Academic Publishers 88/3(2007-03-01), 247-252 0167-6857 88:3<247 2007 88 11240 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer