caa a22 4500 463235797 CHVBK 20180405153256.0 cr unu---uuuuu 170326e20070201xx s 000 0 eng 10.1007/s11240-006-9182-3 doi (NATIONALLICENCE)springer-10.1007/s11240-006-9182-3 Chloroplast genome aberration in micropropagation-derived albino Bambusa edulis mutants, ab1 and ab2 [Elektronische Daten] [Nien-Tsu Liu, Wann-Neng Jane, Hsin-Sheng Tsay, Hui Wu, Wei-Chin Chang, Choun-Sea Lin] Two albino mutants (ab1 and ab2) have been derived from long-term shoot proliferation of Bambusa edulis. Based on transmission electronic microscopy data, the chloroplasts of these mutants were abnormal. To study the mutation of gene regulation in the aberrant chloroplasts, we designed 19 pairs of chloroplast-encoded gene primers for genomic and RT-PCR. Only putative NAD(P)H-quinone oxidoreductase chain 4L (ndhE; DQ908943) and ribosomal protein S7 (rps7; DQ908931) were conserved in both the mutant and wild-type plants. The deletions in the chloroplast genome of these two mutants were different: nine genes were deleted in the chloroplast genomic aberration in ab1 and 11 genes in ab2. The chloroplast genes, NAD(P)H-quinone oxidoreductase chain 4 (ndhD; DQ908944), chloroplast 50S ribosomal protein L14 (rpl14; DQ908934), and ATP synthase beta chain (atpB; DQ908948) were abnormal in both mutants. The gene expressions of 18 of these 20 genes were correlated with their DNA copy number. The two exceptions were: ATP synthase CF0 A chain (atpI; DQ908946), whose expression in both mutants was not reduced even though the copy number was reduced; ribosomal protein S19 (rps19; DQ908949), whose expression was reduced or it was not expressed at all even though there was no difference in genomic copy number between the wild-type and mutant plants. The genomic PCR results showed that chloroplast genome aberrations do occur in multiple shoot proliferation, and this phenomenon may be involved in the generation of albino mutants. Springer Science+Business Media B.V., 2006 Albino mutant nationallicence Bamboo nationallicence Chloroplast genome nationallicence Liu Nien-Tsu Institute of Botany, Academia Sinica, Taipei, Taiwan, Republic of China aut Jane Wann-Neng Institute of Botany, Academia Sinica, Taipei, Taiwan, Republic of China aut Tsay Hsin-Sheng Institute of Biotechnology, Chaoyang University of Technology, Taichung, Taiwan, Republic of China aut Wu Hui Institute of Botany, Academia Sinica, Taipei, Taiwan, Republic of China aut Chang Wei-Chin Institute of Botany, Academia Sinica, Taipei, Taiwan, Republic of China aut Lin Choun-Sea Institute of Botany, Academia Sinica, Taipei, Taiwan, Republic of China aut Plant Cell, Tissue and Organ Culture Kluwer Academic Publishers 88/2(2007-02-01), 147-156 0167-6857 88:2<147 2007 88 11240 https://doi.org/10.1007/s11240-006-9182-3 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11240-006-9182-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Liu Nien-Tsu Institute of Botany, Academia Sinica, Taipei, Taiwan, Republic of China aut NATIONALLICENCE 700 1- Jane Wann-Neng Institute of Botany, Academia Sinica, Taipei, Taiwan, Republic of China aut NATIONALLICENCE 700 1- Tsay Hsin-Sheng Institute of Biotechnology, Chaoyang University of Technology, Taichung, Taiwan, Republic of China aut NATIONALLICENCE 700 1- Wu Hui Institute of Botany, Academia Sinica, Taipei, Taiwan, Republic of China aut NATIONALLICENCE 700 1- Chang Wei-Chin Institute of Botany, Academia Sinica, Taipei, Taiwan, Republic of China aut NATIONALLICENCE 700 1- Lin Choun-Sea Institute of Botany, Academia Sinica, Taipei, Taiwan, Republic of China aut NATIONALLICENCE 773 0- Plant Cell, Tissue and Organ Culture Kluwer Academic Publishers 88/2(2007-02-01), 147-156 0167-6857 88:2<147 2007 88 11240 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer