caa a22 4500 463235800 CHVBK 20180405153256.0 cr unu---uuuuu 170326e20070201xx s 000 0 eng 10.1007/s11240-006-9171-6 doi (NATIONALLICENCE)springer-10.1007/s11240-006-9171-6 High copper levels in the medium improves shoot bud differentiation and elongation from the cultured cotyledons of Capsicum annuum L [Elektronische Daten] [A. Joshi, S. Kothari] The effect of copper sulphate on differentiation and elongation of shoot buds from cotyledonary explants of Capsicum annuum L. cv X-235 was investigated. Shoot buds were induced on medium supplemented with 22.2μM BAP and 14.7μM PAA. Elongation of shoot buds was obtained on MS medium containing 13.3μM BAP+0.58μM GA3. Both shoot induction and elongation media were supplemented with different levels of CuSO4 (0-5μM). The levels of CuSO4 in the induction as well as elongation medium highly influenced the shoot bud formation and their subsequent elongation. Highest number of shoot buds per explant was obtained when the concentration of CuSO4 was increased 30 times to the normal MS level. Shoot buds formation frequency i.e., the number of shoots formed per explant was increased two fold as compared to those formed on control. Elongation both in terms of percentage and length of shoots was better than that on control. Healthy elongated shoots were rooted on MS medium supplemented with 5.7μM IAA. Rooted plantlets were transferred to field conditions. Springer Science+Business Media B.V., 2006 Capsicum annuum nationallicence CuSO4 nationallicence Chilli pepper nationallicence AgNO3 nationallicence Regeneration nationallicence Mineral nutrition nationallicence BAP : 6-benzylaminopurine nationallicence MS : Murashige and Skoog basal medium (1962) nationallicence PAA : Phenyl acetic acid nationallicence GA3 : Gibberellic acid nationallicence Joshi A. Experimental Morphogenesis and Tissue Culture Laboratory, Department of Botany, University of Rajasthan, 302 004, Jaipur, Rajasthan, India aut Kothari S. Experimental Morphogenesis and Tissue Culture Laboratory, Department of Botany, University of Rajasthan, 302 004, Jaipur, Rajasthan, India aut Plant Cell, Tissue and Organ Culture Kluwer Academic Publishers 88/2(2007-02-01), 127-133 0167-6857 88:2<127 2007 88 11240 https://doi.org/10.1007/s11240-006-9171-6 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11240-006-9171-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Joshi A. Experimental Morphogenesis and Tissue Culture Laboratory, Department of Botany, University of Rajasthan, 302 004, Jaipur, Rajasthan, India aut NATIONALLICENCE 700 1- Kothari S. Experimental Morphogenesis and Tissue Culture Laboratory, Department of Botany, University of Rajasthan, 302 004, Jaipur, Rajasthan, India aut NATIONALLICENCE 773 0- Plant Cell, Tissue and Organ Culture Kluwer Academic Publishers 88/2(2007-02-01), 127-133 0167-6857 88:2<127 2007 88 11240 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer