caa a22 4500 463235819 CHVBK 20180405153256.0 cr unu---uuuuu 170326e20070201xx s 000 0 eng 10.1007/s11240-006-9187-y doi (NATIONALLICENCE)springer-10.1007/s11240-006-9187-y Genetic transformation of the medicinal plant Salvia miltiorrhiza by Agrobacterium tumefaciens -mediated method [Elektronische Daten] [Ya-ping Yan, Zhe-zhi Wang] A high-frequency and simple procedure for Agrobacterium tumefaciens-mediated genetic transformation of the medicinal plant Salvia miltiorrhiza was developed. Leaf discs were pre-cultured on MS medium supplemented with 6.6μmoll−1 BAP and 0.5μmoll−1 NAA for one day, then co-cultured with A. tumefaciens strain EHA105 harboring the plasmid pCAMBIA 2301 for three days on the same medium. Regenerated buds were obtained on selection medium (co-culture medium supplemented with 60mgl−1 kanamycin and 200mgl−1 cefotaxime) after two cycles' culture of 10days each and then transferred to fresh MS medium with 60mgl−1 kanamycin for rooting. Fifteen days later, the rooted plantlets were obtained and then successfully transplanted to soil. The transgenic nature of the regenerated plants was confirmed by PCR, Southern hybridization analysis and GUS histochemical assay. Averagely, 1.1 independent verified transgenics per explant plated were obtained through this protocol. Adopting this procedure, positive transformed plants could be obtained within 2-3months from mature seeds germination to transplant to soil, and more than 1,000 transgenic plants with several engineered constructs encoding different genes of interest were produced in our lab in the past two years. Springer Science+Business Media B.V., 2006 Agrobacterium tumefaciens nationallicence Genetic transformation nationallicence Medicinal plant nationallicence Salvia miltiorrhiza nationallicence Secondary metabolic pathway nationallicence Yan Ya-ping Key Laboratory of Ministry of Education for Medicinal Resources and Natural Pharmaceutical Chemistry, College of Life Sciences, Shaanxi Normal University, 710062, Xi'an, Shaanxi, P.R. China aut Wang Zhe-zhi Key Laboratory of Ministry of Education for Medicinal Resources and Natural Pharmaceutical Chemistry, College of Life Sciences, Shaanxi Normal University, 710062, Xi'an, Shaanxi, P.R. China aut Plant Cell, Tissue and Organ Culture Kluwer Academic Publishers 88/2(2007-02-01), 175-184 0167-6857 88:2<175 2007 88 11240 https://doi.org/10.1007/s11240-006-9187-y text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11240-006-9187-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Yan Ya-ping Key Laboratory of Ministry of Education for Medicinal Resources and Natural Pharmaceutical Chemistry, College of Life Sciences, Shaanxi Normal University, 710062, Xi'an, Shaanxi, P.R. China aut NATIONALLICENCE 700 1- Wang Zhe-zhi Key Laboratory of Ministry of Education for Medicinal Resources and Natural Pharmaceutical Chemistry, College of Life Sciences, Shaanxi Normal University, 710062, Xi'an, Shaanxi, P.R. China aut NATIONALLICENCE 773 0- Plant Cell, Tissue and Organ Culture Kluwer Academic Publishers 88/2(2007-02-01), 175-184 0167-6857 88:2<175 2007 88 11240 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer