caa a22 4500 463235827 CHVBK 20180405153256.0 cr unu---uuuuu 170326e20070201xx s 000 0 eng 10.1007/s11240-006-9194-z doi (NATIONALLICENCE)springer-10.1007/s11240-006-9194-z Efficient transient expression and transformation of PEG-mediated gene uptake into mesophyll protoplasts of pepper ( Capsicum annuum L.) [Elektronische Daten] [Joo Jeon, Nam Ahn, Bo Son, Cha Kim, Chang-deok Han, Gun-Do Kim, Sang Gal, Sung-Ho Lee] PEG-mediated transformation was used for gene delivery and evaluation of various parameters affecting the transient expression of a gene for ß-glucuronidase (gus) in mesophyll protoplasts of Capsicum annuum. Transient expression was found to be dependent on PEG concentration and exposure time of plasmid DNA to protoplasts as well as the amount of plasmid DNA. Maximum GUS activity was obtained when protoplasts were applied to 40% concentration and molecular weight was 6,000 of PEG solution with 30min of exposure time. Protoplasts of pepper were transformed with a vector, pCAMBIA::Ac, which contained a pCAMBIA1302 T-DNA vector carrying a maize transposable element, Ac (activator), a selection marker HPT (hygromycin phosphotransferase), and a GFP-coding region driven by the 35S promoter in the presence of PEG. Approximately 30% of the protoplasts expressed GFP. Visibly transformed colonies were obtained from protoplasts after 2months of culture and GFP was expressed. Southern hybridization confirmed the presence of Ac in the pepper genome. Springer Science+Business Media B.V., 2007 Ac Transposable element nationallicence Capsicum annuum L nationallicence Protoplast transformation nationallicence Transient expression nationallicence PEG : polyethylene glycol nationallicence PVP : polyvinylpyrrolidone nationallicence Jeon Joo Division of Applied Life Science (BK21) and Environmental Biotechnology National Core Research Center, Gyeongsang National University, 660-701, Jinju, Korea aut Ahn Nam Division of Applied Life Science (BK21) and Environmental Biotechnology National Core Research Center, Gyeongsang National University, 660-701, Jinju, Korea aut Son Bo Division of Applied Life Science (BK21) and Environmental Biotechnology National Core Research Center, Gyeongsang National University, 660-701, Jinju, Korea aut Kim Cha Division of Applied Life Science (BK21) and Environmental Biotechnology National Core Research Center, Gyeongsang National University, 660-701, Jinju, Korea aut Han Chang-deok Division of Applied Life Science (BK21) and Environmental Biotechnology National Core Research Center, Gyeongsang National University, 660-701, Jinju, Korea aut Kim Gun-Do Department of Microbiology, College of Natural Science, Pukyong National University, 608-737, Busan, Korea aut Gal Sang Department of Microbiological Engineering, Jinju National University, 660-758, Jinju, Korea aut Lee Sung-Ho Division of Applied Life Science (BK21) and Environmental Biotechnology National Core Research Center, Gyeongsang National University, 660-701, Jinju, Korea aut Plant Cell, Tissue and Organ Culture Kluwer Academic Publishers 88/2(2007-02-01), 225-232 0167-6857 88:2<225 2007 88 11240 https://doi.org/10.1007/s11240-006-9194-z text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11240-006-9194-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Jeon Joo Division of Applied Life Science (BK21) and Environmental Biotechnology National Core Research Center, Gyeongsang National University, 660-701, Jinju, Korea aut NATIONALLICENCE 700 1- Ahn Nam Division of Applied Life Science (BK21) and Environmental Biotechnology National Core Research Center, Gyeongsang National University, 660-701, Jinju, Korea aut NATIONALLICENCE 700 1- Son Bo Division of Applied Life Science (BK21) and Environmental Biotechnology National Core Research Center, Gyeongsang National University, 660-701, Jinju, Korea aut NATIONALLICENCE 700 1- Kim Cha Division of Applied Life Science (BK21) and Environmental Biotechnology National Core Research Center, Gyeongsang National University, 660-701, Jinju, Korea aut NATIONALLICENCE 700 1- Han Chang-deok Division of Applied Life Science (BK21) and Environmental Biotechnology National Core Research Center, Gyeongsang National University, 660-701, Jinju, Korea aut NATIONALLICENCE 700 1- Kim Gun-Do Department of Microbiology, College of Natural Science, Pukyong National University, 608-737, Busan, Korea aut NATIONALLICENCE 700 1- Gal Sang Department of Microbiological Engineering, Jinju National University, 660-758, Jinju, Korea aut NATIONALLICENCE 700 1- Lee Sung-Ho Division of Applied Life Science (BK21) and Environmental Biotechnology National Core Research Center, Gyeongsang National University, 660-701, Jinju, Korea aut NATIONALLICENCE 773 0- Plant Cell, Tissue and Organ Culture Kluwer Academic Publishers 88/2(2007-02-01), 225-232 0167-6857 88:2<225 2007 88 11240 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer