caa a22 4500 463235851 CHVBK 20180405153256.0 cr unu---uuuuu 170326e20070201xx s 000 0 eng 10.1007/s11240-006-9180-5 doi (NATIONALLICENCE)springer-10.1007/s11240-006-9180-5 In Vitro Propagation Of Four Watsonia Species [Elektronische Daten] [Glendon Ascough, John Erwin, Johannes van Staden] The genus Watsonia has a number of species with potential to be developed as new ornamental crop plants, but to date there are no reports on in vitro propagation of any member of this genus. Seeds from four Watsonia species, Watsonia gladioloides, Watsonia lepida, Watsonia laccata, and Watsonia vanderspuyiae were decontaminated and germinated on one-tenth strength MS media without hormones or sucrose. Shoots were induced from seedling hypocotyl segments when both an auxin [α-naphthaleneacetic acid (NAA)] and cytokinin [N6−benzylaminopurine (BA)] were present in the medium, while root and leaf explants failed to produce shoots. Multiplication of axillary shoots was greatest when only BA (0.5mgl−1) was added to the medium. Shoot explants propagated in a ‘liquid-shake' culture exhibited greater growth rates than those on agar-solidified medium, but shoot production varied between species. Meristemoids were induced in all species, but no significant trend was found between growth index (GI) and meristemoid formation, suggesting that reduction in GI may not necessarily be a prerequisite for producing meristemoids. Corm formation was inconsistent and storage organs could only be induced in one of the four species, W. vanderspuyiae. This occurred best at 25°C with 3% sucrose and an agar level of 15%. Indole-3-acetic acid (IAA) and NAA at 1mgl−1 significantly increased mean number of roots per shoot explant on all four species, while indole-3-butyric acid (IBA) was more effective when applied at 0.1mgl−1. Plantlet survival ex vitro was negatively affected when NAA and 2,4-dichlorophenoxyacetic acid (2,4-D) were used to root shoot explants for all species. In W. laccata, all auxin treatments [IAA, IBA, NAA, phenylacetic acid (PAA), and 2,4-D] at a concentration of 1mgl−1 significantly reduced ex vitro survival of plantlets. Successful micropropagation of Watsonia is an important step in the further development of this genus as a horticultural crop. Springer Science+Business Media B.V., 2006 Acclimatization nationallicence Corm formation nationallicence Iridaceae nationallicence Liquid culture nationallicence rooting nationallicence 2,4-D : 2,4-dichlorophenoxyacetic acid nationallicence AC : activated charcoal nationallicence ANOVA : analysis of variance nationallicence BA : N6-benzylaminopurine nationallicence GI : growth index nationallicence IAA : indole-3-acetic acid nationallicence IBA : indol-3-butyric acid nationallicence MS : Murashige and Skoog basal medium nationallicence NAA : α-naphthaleneacetic acid nationallicence PAA : phenylacetic acid nationallicence PP3 : paclobutrazol nationallicence Ascough Glendon Research Centre for Plant Growth and Development, School of Biological and Conservation Sciences, University of KwaZulu-Natal, Private Bag X01, 3209, Pietermaritzburg, Scottsville, South Africa aut Erwin John Department of Horticultural Science, University of Minnesota, 1970 Folwell Ave., 55108, St Paul, MN, USA aut van Staden Johannes Research Centre for Plant Growth and Development, School of Biological and Conservation Sciences, University of KwaZulu-Natal, Private Bag X01, 3209, Pietermaritzburg, Scottsville, South Africa aut Plant Cell, Tissue and Organ Culture Kluwer Academic Publishers 88/2(2007-02-01), 135-145 0167-6857 88:2<135 2007 88 11240 https://doi.org/10.1007/s11240-006-9180-5 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11240-006-9180-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ascough Glendon Research Centre for Plant Growth and Development, School of Biological and Conservation Sciences, University of KwaZulu-Natal, Private Bag X01, 3209, Pietermaritzburg, Scottsville, South Africa aut NATIONALLICENCE 700 1- Erwin John Department of Horticultural Science, University of Minnesota, 1970 Folwell Ave., 55108, St Paul, MN, USA aut NATIONALLICENCE 700 1- van Staden Johannes Research Centre for Plant Growth and Development, School of Biological and Conservation Sciences, University of KwaZulu-Natal, Private Bag X01, 3209, Pietermaritzburg, Scottsville, South Africa aut NATIONALLICENCE 773 0- Plant Cell, Tissue and Organ Culture Kluwer Academic Publishers 88/2(2007-02-01), 135-145 0167-6857 88:2<135 2007 88 11240 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer