caa a22 4500 46323586X CHVBK 20180405153256.0 cr unu---uuuuu 170326e20070201xx s 000 0 eng 10.1007/s11240-006-9192-1 doi (NATIONALLICENCE)springer-10.1007/s11240-006-9192-1 Changes in phytochelatins and their biosynthetic intermediates in red spruce ( Picea rubens Sarg.) cell suspension cultures under cadmium and zinc stress [Elektronische Daten] [P. Thangavel, Stephanie Long, Rakesh Minocha] Cell suspension cultures of red spruce (Picea rubens Sarg.) were selected to study the effects of cadmium (Cd) and zinc (Zn) on phytochelatins (PCs) and related metabolites after 24h exposure. The PC2 and its precursor, γ-glutamylcysteine (γ-EC) increased two to fourfold with Cd concentrations ranging from 12.5 to 200μM as compared to the control. However, Zn-treated cells showed a less than twofold increase in γ-EC and PC2 levels as compared to the control even at the highest concentration of 800μM. In addition, unidentified higher chain PCs were also found in both the Cd and Zn treated cells and they increased significantly with increasing concentrations of Cd and Zn. The cellular ratio of PC2:Cd or Zn content clearly indicated that Cd (with ratios ranging from 0.131 to 0.546) is a more effective inducer of PC2 synthesis/accumulation than Zn (with ratios ranging from 0.032 to 0.102) in red spruce cells. A marginal decrease in glutathione (GSH) was observed in both Cd and Zn treated cells. However, the GSH precursor, cysteine, declined twofold with all Cd concentrations while the decrease with Zn was 1.5-2-fold only at the higher treatment concentrations of Zn as compared to control. In addition, changes in other free amino acids, polyamines, and inorganic ions were also studied. These results suggest that PCs and their biosynthetic intermediates play a significant role in red spruce cells protecting against Cd and Zn toxicity. Springer Science+Business Media B.V., 2006 Amino acids nationallicence Cysteine nationallicence γ-Glutamylcysteine nationallicence Glutathione nationallicence Heavy metals nationallicence HPLC nationallicence Inorganic ions nationallicence Metal detoxification nationallicence Polyamines nationallicence ACN : Acetonitrile nationallicence γ-EC : γ-glutamylcysteine nationallicence GSH : Glutathione nationallicence HPLC : High-performance liquid chromatography nationallicence mBBr : Monobromobimane nationallicence NAC : N-acetyl-l-cysteine nationallicence PCs : Phytochelatins nationallicence TFA : Trifluroacetic acid nationallicence Thangavel P. Northern Research Station, USDA Forest Service, 271 Mast Road, 03824, Durham, NH, USA aut Long Stephanie Northern Research Station, USDA Forest Service, 271 Mast Road, 03824, Durham, NH, USA aut Minocha Rakesh Northern Research Station, USDA Forest Service, 271 Mast Road, 03824, Durham, NH, USA aut Plant Cell, Tissue and Organ Culture Kluwer Academic Publishers 88/2(2007-02-01), 201-216 0167-6857 88:2<201 2007 88 11240 https://doi.org/10.1007/s11240-006-9192-1 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11240-006-9192-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Thangavel P. Northern Research Station, USDA Forest Service, 271 Mast Road, 03824, Durham, NH, USA aut NATIONALLICENCE 700 1- Long Stephanie Northern Research Station, USDA Forest Service, 271 Mast Road, 03824, Durham, NH, USA aut NATIONALLICENCE 700 1- Minocha Rakesh Northern Research Station, USDA Forest Service, 271 Mast Road, 03824, Durham, NH, USA aut NATIONALLICENCE 773 0- Plant Cell, Tissue and Organ Culture Kluwer Academic Publishers 88/2(2007-02-01), 201-216 0167-6857 88:2<201 2007 88 11240 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer